摘要
核酸检测方法可快速鉴定特异基因指标,但其广泛应用受限于多种仪器设备串行使用以及对操作人员的高专业技术要求。本团队开发了一套注射式微流控芯片全集成核酸分析系统,该系统主要包含两大模块,分别是可以为不同类型临床样本提供多种核酸提取方法的全自动注射式核酸提取模块,以及基于微流控芯片的微纳体系多指标联合并行检测等温扩增核酸检测模块。这两大模块既可以单独发挥各自的功能,也可以组合成全集成注射式微流控芯片核酸分析系统,形成全集成自动化、微纳反应体系、快速、多指标联合并行检测的核酸检测分析平台。采用本团队开发的注射式微流控芯片全集成核酸分析系统,分别对热带念珠菌标准株培养菌液和64例外阴阴道念珠菌感染疾病的临床拭子样本进行检测。结果显示:本系统对菌液的最低检测限为3.95×102 CFU/mL,而且样品制备更方便快捷,仅需1次加样操作,核酸提取时间为10 min;64例临床样本检测效果与金标准培养法相比,卡方检验为1,Kappa值为0.950,说明两种方法无显著差异,且一致性很高。本团队开发的注射式微流控芯片全集成核酸分析系统,可以为临床多指标微纳体系核酸快速检测提供一个可靠的平台,为临床医疗应用提供精准快检技术与便捷分析仪器支撑。
Objective Nucleic acid detection methods enable the rapid identification of specific genetic indicators.However,their extensive application is limited by the sequential use of multiple instruments and the high technical requirements for operators.A fully integrated nucleic acid analysis system can automate the sample-to-answer detection process.However,nucleic acid amplification via PCR technology is time-consuming,and stringent requirements are placed on temperature control accuracy and heating/cooling speed,which increases the manufacturing cost of the corresponding instruments.These systems also lack the flexibility to accommodate variations in sample preprocessing methods and nucleic acid extraction protocols for different types of clinical samples.To address these challenges,we present a novel,fully integrated nucleic acid analysis system that contains a syringe-based sample processing and nucleic acid extraction module,a fully enclosed PC-based multiplexed detection microfluidic chip,and a nucleic acid amplification product detection module.We applied this system for the precise medical testing of various pathogenic infections in gynecology.The results indicate that our system can achieve precise medical molecular diagnosis with the fully integrated automation of a micro-nano reaction system,as well as speedy processing,high sensitivity and specificity,and multiplexed parallel detection.The system also shows potential applications in the in vitro identification of genetic and other nucleic acid-related diseases,holding significant social and economic value for the prevention and control of major diseases in China.Methods This paper presents a fully integrated nucleic acid analysis system based on a syringe microfluidic chip.The system consists of two modules.The first is an automated syringe nucleic acid extraction module that accommodates multiple nucleic acid extraction techniques based on different clinical sample types.This process involves four primary steps:sample loading,incubation,washing,and lysis
作者
黄琴
黄乐阳
靳翔宇
毛则尹
邓安妮
吕文琦
钟豪
王铈弘
石艺璇
陈家辉
黄世光
黄国亮
Huang Qin;Huang Leyang;Jin Xiangyu;Mao Zeyin;Deng Anni;Lu Wenqi;Zhong Hao;Wang Shihong;Shi Yixuan;Chen Jiahui;Huang Shiguang;Huang Guoliang(Department of Biomedical Engineering,School of Medicine,Tsinghua University,Beijing 100084,China)
出处
《中国激光》
EI
CAS
CSCD
北大核心
2024年第9期190-199,共10页
Chinese Journal of Lasers
基金
国家自然科学基金(61927819,81827808,62105177,62375148)
国家重点研发计划(2018YFA0704000)
四川省科技计划重点项目(2021YFO0060,2022YFS0637)
北京实验室基金与清华大学自主科研基金(20194180031,20201080058,2020108510)
万科公共卫生与健康学科发展专项基金(2022Z82WKJ002)。
关键词
医用光学
注射式
微流控芯片
全集成核酸分析系统
精准医疗应用
medical optics
syringe
microfluidic chip
fully integrated nucleic acid analysis system
application of precision medicine
