摘要
目的探讨血清血管生成素样蛋白8(ANGPTL8)、凝集蛋白-1(ITLN-1)对川崎病(KD)患儿冠状动脉损伤的预测价值。方法选取2020年5月至2023年5月在本院就诊的108例KD患儿作为研究组,根据是否发生冠状动脉损伤分为冠状动脉损伤组(n=37)与无冠状动脉损伤组(n=71);另选取同期体检健康儿童72例作为对照组。采用双抗体夹心-酶联免疫吸附法检测所有受试者血清炎症相关指标及ANGPTL8、ITLN-1水平;Pearson法分析冠状动脉损伤KD患儿血清ANGPTL8、ITLN-1水平与炎症指标的相关性;评估血清ANGPTL8、ITLN-1水平对KD患儿冠状动脉损伤的预测价值。结果冠状动脉损伤组KD患儿PLT、CRP、IL-6和TNF-α水平明显高于无冠状动脉损伤组及对照组(F/χ^(2)值分别为67.198、75.056、71.876及94.158,P<0.05);与对照组相比,研究组KD患儿血清ANGPTL8水平明显升高(t=17.490,P<0.05),ITLN-1水平明显降低(t=13.162,P<0.05);与无冠状动脉损伤组相比,冠状动脉损伤组患儿血清ANGPTL8水平明显升高(t=7.454,P<0.05),ITLN-1水平明显降低(t=7.631,P<0.05)。Pearson相关分析结果显示,冠状动脉损伤KD患儿血清ANGPTL8水平与PLT及CRP、IL-6、TNF-α水平均呈正相关(r=0.514、0.638、0.484、0.674,P<0.05),ITLN-1水平与PLT及CRP、IL-6、TNF-α水平均呈负相关(r=-0.603、-0.659、-0.439、-0.646,P<0.05)。ROC曲线分析结果显示,血清ANGPTL8、ITLN-1联合预测KD患儿冠状动脉损伤的曲线下面积(AUC)为0.950,敏感度为89.19%,特异度为85.91%。多因素Logistic回归分析结果显示,PLT、CRP、IL-6、TNF-α、ANGPTL8水平升高是KD患儿冠状动脉损伤的独立危险因素,ITLN-1水平升高是KD患儿冠状动脉损伤的保护因素。其OR值及95%CI分别为1.623(1.088~2.421)、1.722(1.266~2.342)、1.413(1.035~1.930)、1.725(1.210~2.460)、1.534(1.114~2.111)和0.685(0.485~0.967)。结论KD冠状动脉损伤患儿血清ANGPTL8水平明显升高,ITLN-1水平明显降低,且ANGPTL8水平升高、ITLN-1水平降
Objective To explore the predictive value of serum angiopoietin-like protein 8(ANGPTL8)and intelectin-1(ITLN-1)in coronary artery injury in children with Kawasaki disease(KD).Methods A total of 108 children with KD who were admitted to our hospital from May 2020 to May 2023 were regarded as the study group,they were divided into coronary artery injury group(n=37)and non-coronary artery injury group(n=71)according to the occurrence of coronary artery injury;another 72 healthy children who underwent physical examination were regarded as the control group.Double-antibody sandwich enzyme-linked immunosorbent assay was applied to detect serum inflammation related markers and levels of ANGPTL8 and ITLN-1 in all subjects.Pearson s method was applied to analyze the correlation between serum levels of ANGPTL8,ITLN-1 and inflammatory markers in KD patients with coronary artery injury.The predictive value of serum ANGPTL8 and ITLN-1 levels for coronary artery injury in children with KD was evaluated.Results In the coronary artery injury group,KD children had significantly higher levels of platelet(PLT),C-reaction protein(CRP),interleukin-6(IL-6)and tumor necrosis factor alpha(TNF-α)compared to the non-coronary artery injury group(F/χ^(2)=67.198,75.056,71.876 and 94.158,respectively,all P<0.05);compared with the control group,KD children in the study group showed a significant increase in serum ANGPTL8 levels(t=17.490,P<0.05),and a significant decrease in ITLN-1 levels(t=13.162,P<0.05);compared with the non-coronary artery injury group,KD children in the coronary artery injury group exhibited a significant increase in serum ANGPTL8 levels(t=7.454,P<0.05)and a significant decrease in ITLN-1 levels(t=7.631,P<0.05).The results of pearson correlation analysis showed that in KD children with coronary artery injury,serum levels of ANGPTL8 were positively correlated with PLT,CRP,IL-6 and TNF-αlevels(r=0.514,0.638,0.484 and 0.674,respectively,P<0.05),while the ITLN-1 levels were negatively correlated with PLT,CRP,IL-6 and TNF-αl
作者
梁丹丹
张欣
裴新昕
LIANG Dandan;ZHANG Xin;PEI Xinxin(Department of Infectious diseases,Xuzhou Children s Hospital,Jiangsu Xuzhou 221000,China)
出处
《中国妇幼健康研究》
2024年第7期38-43,共6页
Chinese Journal of Woman and Child Health Research
基金
盐城市科学技术局2022年度市重点研发计划项目(YCBE202226)。
