摘要
目的:建立差示扫描量热法(DSC)鉴别区分天然没药、胶质没药、没药炮制品、没药混伪品。方法:收集不同基原、炮制品、混伪品的没药样品,通过考察升温范围、升温速率、粒度大小等因素对没药DSC试验条件的影响,在优化条件下应用差示扫描量热仪对没药各样品进行差热图谱扫描、对比分析。结果:DSC试验优化条件为升温范围-25~550℃、粒度大小100目、升温速率50℃/min;130℃附近的吸热峰与330℃附近的放热峰为没药的特征峰。天然没药、胶质没药在130℃和330℃位置特征峰的平均温度、热焓值均有明显差异,天然没药在两位置的热焓值均明显低于胶质没药。通过比对特征峰可以清晰地鉴别出伪品(松脂、枫香脂)在130℃和330℃附近位置均无该特征峰,混伪品枫香脂在10℃有吸热特征峰,混伪品松脂在150℃附近有明显的放热峰;醋制天然没药在180℃与240℃附近特征峰的热焓值均比天然没药低。结论:DSC试验可快速准确鉴别天然没药、胶质没药、伪品枫香脂及松脂;DSC可快速区分醋制没药与没药;130℃吸热峰、330℃放热峰为没药特征峰。
Objective:To establish the differential scanning calorimetry(DSC)for distinguishing natural Moyao(Myrrha),colloidal Moyao,processed Moyao and its counterfeit species.Methods:The samples of Moyao from different medicinal sources,processed products and counterfeit species were collected.By investigating the effect of heating range,heating rate,particle size and other factors on the DSC test con-ditions of Moyao,the differential thermal map of each sample of Moyao was scanned and analyzed by DSC under the optimized conditions.Results:The optimized DSC test conditions were as follows:the heating range of-25 to 550℃,the particle size of 100 mesh,and the heating rate of 50℃/min.The endothermic peak near 130℃and the exothermic peak near 330℃were the characteristic peaks of Moyao.The average temperature and enthalpy values of characteristic peaks at 130℃and 330℃of natural Moyao and colloid Moyao were significantly different,and the enthalpy values of natural Moyao were significantly lower than those of colloid Moyao.The counterfeit species[turpentine,Fengxiangzhi(Liquidambaris Resina)]could be clearly identified through comparing the characteristic peaks,which had no characteristic peak near 130℃and 330℃.There was an endothermic peak at 10℃for the counterfeit Fengxiangzhi,and an obvious exothermic peak near 150℃for the counterfeit turpentine.The enthalpy values of characteristic peaks near 180℃and 240℃of natural Moyao processed with vinegar were lower than those of natural Moyao.Conclusions:DSC test could quickly and accurately distinguish natural Moyao,colloid Moyao,counterfeit Fengxiangzhi and turpentine,and could quickly distinguish Moyao processed with vinegar and Moyao.Endothermic peak at 130℃and exothermic peak at 330℃were the characteristic peaks of Moyao.
作者
苏玉纯
沈紧治
谢素治
田婧
SU Yuchun;SHEN Jinzhi;XIE Suzhi;TIAN Jing(Department of Pharmacy,Zhongshan Hospital Affiliated to Xiamen University,Xiamen 361004,China;Department of Pharmacy,Xiamen Haicang Hospital,Xiamen 361026,China;Pharmaceutical Factory,Tongrentang Technology Development Co.,Ltd.,Beijing 100075,China)
出处
《山东中医药大学学报》
2024年第4期478-484,共7页
Journal of Shandong University of Traditional Chinese Medicine
基金
福建省自然科学基金项目(编号:2021J05276)
厦门市医疗卫生指导性项目(编号:3502Z20199027)。
关键词
天然没药
胶质没药
混伪品
枫香脂
松脂
差示扫描量热法
鉴别研究
natural Moyao(Myrrha)
colloidal Moyao(Myrrha)
counterfeit species
Fengxiangzhi(Liquidambaris Resina)
turpentine
differential scanning calorimetry
identification study
