摘要
目的了解青海省隆宝滩地区棘球绦虫虫卵的环境污染情况,为有针对性地制定棘球蚴病防治措施提供依据。方法2023年3—4月,在青海省玉树市隆宝镇措桑村的养犬户家庭、村内主要街道及野外区域,分别采集犬科动物粪便、动物毛发、水、土壤、草和食物等不同类型的环境样本,利用PCR技术检测样本棘球绦虫虫卵并比较检出率差异。采用ArcGIS软件并结合ASTER GDEM高程数据,制作样本分布高程地图。结果共采集环境样本400份,棘球绦虫虫卵总检出率为13.50%(54/400);其中犬科动物粪便、动物毛发、水、土壤、草、食物的检出率分别为30.43%(42/138)、5.33%(4/75)、15.38%(2/13)、5.26%(6/114)、0(0/37)、0(0/23)。138份犬科动物粪便中,19份野外粪便未能通过PCR鉴定出动物宿主;其余119份样本中,犬、藏狐、赤狐粪便虫卵检出率分别为11.29%(7/62)、48.84%(21/43)、50.00%(7/14),差异有统计学意义(χ^(2)=20.481,P<0.05)。138份犬科动物粪便中,细粒棘球绦虫、多房棘球绦虫和石渠棘球绦虫虫卵检出率分别为2.17%(3/138)、18.12%(25/138)和16.67%(23/138),差异有统计学意义(χ^(2)=19.858,P<0.05)。75份动物毛发样本中,犬、牛的毛发检出率分别为6.06%(4/66)、0(0/9),差异无统计学意义(P>0.05)。13份水样本中,野外河流溪水、村内井水和村内积水的虫卵检出率分别为20.00%(2/10)、0(0/2)和0(0/1),差异无统计学意义(P>0.05)。114份土壤样本中,养犬户家庭、村内主要街道和野外土壤虫卵检出率分别为15.00%(3/20)、15.00%(3/20)和0(0/74),差异有统计学意义(P<0.05)。动物毛发、水和土壤样本中检出的虫卵均为细粒棘球绦虫。在虫卵分布方面,检出虫卵的犬科动物粪便高度集中于野外道路附近地域,其他环境检出虫卵的样本无明显集中趋势。结论青海隆宝滩地区环境虫卵污染严重且分布广泛,需继续采取犬(特别是未拴养犬)及野外传染源(狐狸)驱虫等�
Objective To investigate the environmental contamination status of Echinococcus eggs in Longbaotan area of Qinghai Province for evidence to formulate targeted measures in controlling echinococcosis.Methods From March to April 2023,we collected different types of environmental samples,including canine feces,animal hair,water,soil,grass and food,from the dog households,main streets in the villages and wild areas.PCR technology was used to detect Echinococcus eggs in the samples from different sources,and the detection rate of eggs was compared.ArcGIS software combined with ASTER GDEM elevation data was used to make the elevation map of sample distribution.Results A total of 400 environmental samples were harvested.The total detection rate of Echinococcus eggs was 13.50%(54/400).Of the samples obtained,138 were canine feces,75 animal hair,13 water,114 soil,37 grass and 23 food,in which the detection rate of Echinococcus eggs was 30.43%(42/138),5.33%(4/75),15.38%(2/13),5.26%(6/114),0(0/37)and 0(0/23),respectively.Among 138 aliquots of canine faeces,the animals hosts were failed to be identified in the 19 aliquots of wild canine feces by PCR.Of the other 119 samples,the detection rates of Echinococcus egg in the feces of dog,Tibetan fox and red fox were 11.29%(7/62),48.84%(21/43)and 50.00%(7/14),respectively,with significant difference(χ^(2)=20.481,P<0.05).The egg detection rates of Echinococcus granulosus,E.multilocularis and E.shiquicus were 2.17%(3/138),18.12%(25/138)and 16.67%(23/138),respectively,with significant difference(χ^(2)=19.858,P<0.05).In the 75 animal hair samples,the detection rates of Echinococcus egg in dog hair and cow hair were 6.06%(4/66)and 0(0/9),respectively,with no significant difference(P>0.05).Of the 13 water samples,the detection rates of Echinococcus egg in wild river and streams,village well water and stagnant water were 20.00%(2/10),0(0/2)and 0(0/1),respectively,with no significant difference(P>0.05).Among the 114 soil samples,the detection rates of Echinococcus egg in dog households,m
作者
孙晨清
杨诗杰
韩帅
王旭
陈军虎
洪炀
张颋
张仁杰
贺耿城
马霄
赵存哲
龚春花
王吉鹏
周晓农
SUN Chenqing;YANG Shijie;HAN Shuai;WANG Xu;CHEN Junhu;HONG Yang;ZHANG Ting;ZHANG Renjie;HE Gengcheng;MAXiao;ZHAO Cunzhe;GONG Chunhua;WANG Jipeng;ZHOU Xiaonong(National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research),National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,National Health Commission Key Laboratory on Parasite and Vector Biology,WHO Collaborating Centre for Tropical Diseases,National Center for International Research on Tropical Diseases,Ministry of Science and Technology,Shanghai 200025,China;Qinhai Institute for Endemic Disease Prevention and Control;Yushu Center for Disease Control and Prevention;School of Life Science,Fudan University)
出处
《热带病与寄生虫学》
CAS
2024年第3期164-171,共8页
Journal of Tropical Diseases and Parasitology
基金
国家重点研发计划项目(2021YFC2300800)。
关键词
棘球绦虫
棘球蚴病
虫卵
环境
污染
隆宝滩
Echinococcus
Echinococcosis
Eggs
Environment
Contaminate
Longbaotan
