摘要
【背景】载体类型对微生物肥料的应用效果和成本有显著影响。【目的】筛选吸附贝莱斯芽孢杆菌(Bacillus velezensis) PZ-3能力强的载体。【方法】以生物炭、腐殖酸、生物质灰渣、有机肥、菌糠和稻壳粉作为载体原料,以贝莱斯芽孢杆菌PZ-3为试验菌株与载体进行吸附试验,通过稀释涂布-菌落计数法,流式细胞术及玉米盆栽试验,筛选与目标菌株适配的载体,并探究其吸附目标菌株后对玉米根际土壤中微生物多样性的影响。【结果】腐殖酸+菌糠吸附目标菌株后在玉米根际土壤中定殖效果最佳,根际土壤中目标菌株的数量在第7天达到最高,为1.28×10^(5) CFU/g,其相对丰度为59.15%;该处理与原始土壤相比,玉米的株高、茎粗、地上物质干重提升了28.51%、19.05%、48.65%;且该处理显著提升(P<0.05)玉米根际土壤中链霉菌属(Streptomyces)、假单胞菌属(Pseudomonas)的相对丰度,显著降低了索氏梭菌属(Paeniclostridium)、瓶毛壳属(Lophotrichus)、赤霉菌属(Gibberella)的相对丰度。【结论】腐殖酸+菌糠吸附贝莱斯芽孢杆菌PZ-3的能力强,联合配施对玉米促生效果明显,且显著提升玉米根际土壤中植物促生菌的相对丰度,降低植物病原真菌的相对丰度。
[Background]Carriers influences the effects and costs of microbial fertilizers.[Objective]To screen the vector with strong adsorption ability of Bacillus velezensis PZ-3.[Methods]We carried out adsorption experiments for B.velezensis PZ-3 with biochar,humic acid,biomass ash,organic fertilizer,mushroom bran,and rice husk powder as carriers.The carrier suitable for the target strain was screened by dilution coating-colony counting method,flow cytometry,and maize pot experiment.Then,we investigated the effects of the adsorbed target strain on the microbial diversity in the rhizosphere soil of maize.[Results]The best colonization effect was achieved in the rhizosphere soil of maize after the adsorption of the target strain by humic acid+mushroom bran.The abundance and number of the target strain in the rhizosphere soil of this treatment peaked at 1.28×10^(5) CFU/g,with a relative abundance of 59.15%,on day 7.Compared with the control(original soil),this treatment increased the plant height,stem diameter,and aboveground dry weight of maize by 28.51%,19.05%,and 48.65%,respectively.Moreover,this treatment increased the relative abundance of Streptomyces and Pseudomonas(P<0.05)and reduced the relative abundance of Paeniclostridium,Lophotrichus,and Gibberella in the rhizosphere soil of maize.[Conclusion]Humic acid combined with mushroom bran had a strong ability to adsorb B.velezensis PZ-3.The combination significantly promoted the growth of maize,increased the relative abundance of plant growth-promoting strains,and reduced the relative abundance of plant pathogenic fungi in the rhizosphere soil of maize.
作者
窦鑫
郭鹏博
张伟
王敬红
陈昭
洪艳华
孙冬梅
王伟东
DOU Xin;GUO Pengbo;ZHANG Wei;WANG Jinghong;CHEN Zhao;HONG Yanhua;SUN Dongmei;WANG Weidong(21 Key Laboratory of Low-Carbon Green Agriculture in Northeast Plain,Ministry of Agriculture and Rural Affairs,College of Life Science and Biotechnology,Heilongjiang Bayi Agricultural University,Daqing 163319,Heilongjiang,China;Key Laboratory of Saline-Alkali Vegetation Ecology Restoration,Ministry of Education,College of Life Science,Northeast Forestry University,Harbin 150040,Heilongjiang,China;College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing 163319,Heilongjiang,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2024年第6期2013-2029,共17页
Microbiology China
基金
国家重点研发计划(2023YFD1500501-01)
黑龙江省揭榜挂帅科技攻关项目(2022ZXJ08B01)
国家自然科学基金区域创新发展联合基金(U22A20444)
中央高校基本科研业务费专项资金(2572023CT08)
黑龙江八一农垦大学三纵计划(ZDZX202105)。
关键词
贝莱斯芽孢杆菌
载体
玉米
根际微生物
微生物多样性
Bacillus velezensis
carrier
maize
rhizosphere microorganisms
microbial diversity
