摘要
木棉是木棉科木棉属落叶乔木植物,是我国南方重要的观赏植物,是广州市和攀枝花市的市花,不仅具有较高的观赏价值,还具有有较高的经济价值、药用价值和食用价值。木棉传统的繁殖方式为播种和嫁接繁殖,其种子不易保存、易变质从而降低种子萌发率;而嫁接繁殖成活率更低。因此,本试验开展木棉组织培养快繁技术研究,以木棉种子为试验材料,通过开展对木棉种子不同消毒方法试验,确定最适合木棉种子的消毒方法,获得无菌种苗;并以无菌种苗为外植体,开展不同基础培养基、顶芽诱导激素组合、顶芽和丛生芽诱导和增殖培养基、生根培养基的筛选及炼苗试验,探索最佳基础培养基、激素组合、诱导培养基和增殖培养基、生根培养基及炼苗方法。结果表明:木棉组织培养最佳基础培养基为MS+蔗糖30 g/L+卡拉胶7.5 g/L;木棉种子最佳消毒方法为A_(2):75%乙醇消毒1 min→无菌水清洗5次→50%H_(2)O_(2)溶液消毒4 h→无菌水清洗5次;最佳木棉种子苗顶芽诱导分化幼芽的植物生长调节剂为6-BA+NAA的组合;最佳诱导及增殖培养基为C_(2):MS+6-BA 0.5 mg/L+IBA 0.5 mg/L+蔗糖30 g/L+卡拉胶7.5 g/L;最佳生根培养基为E_(3):1/2 MS+IBA 0.5 mg/L+蔗糖30 g/L+卡拉胶7.5 g/L+活性炭0.2 g/L;最佳炼苗基质为沙子∶泥炭土∶蛭石=1∶1∶1。通过对木棉种子消毒方法、培养基的筛选、激素组合及炼苗试验,对木棉组织培养技术进行优化,并建立了高效木棉组织培养技术体系,为木棉种苗离体保存、优良单株扩繁、健康种苗繁育及工厂化育苗提供技术支持,也可为木棉遗传转化体系奠定基础。
Bombax ceiba is a deciduous tree belonging to the Genus Bombax in the Malvaceae family.It holds significant importance as an ornamental plant of economic,medicinal and edible value in southern China.It is the city flower of Guangzhou and Panzhihua.The traditional propagation methods for B.ceiba are seed sowing and grafting.However,the seeds are difficult to preserve and easily deteriorate,leading to decreased germination rates.Grafting results in even lower survival rates.Therefore,this experiment aimed to study the tissue culture techniques for the rapid propagation of B.ceiba.In this study,B.ceiba seeds were used as the experimental materials,and different disinfection methods were tested to determine the most suitable method for seed disinfection,aiming to obtain sterile seedlings.Using the sterile seedlings as the explants,various basic culture media,combinations of shoot-inducing hormones,induction and proliferation media for apical and adventitious buds,as well as rooting media,were tested and optimized.Additionally,seedling acclimatization experiments were conducted to explore the optimal basic culture medium,hormone combination,induction and proliferation medium,rooting medium,and seedling acclimatization methods.The results showed that the optimal basic culture medium for B.ceiba tissue culture was MS medium supplemented with 30 g/L sucrose and 7.5 g/L agar.The best disinfection method for B.ceiba seeds was 75% ethanol treatment for 1 min,followed by five rinses with sterile water,4-hour sterilization with a 50%H_(2)O_(2) solution prepared from 30%H_(2)O_(2),and another five rinses with sterile water.The optimal plant growth regulators for inducing differentiation of apical buds into young shoots were a combination of 6-benzylaminopurine(6-BA)and naphthaleneacetic acid(NAA).The best induction and proliferation medium was MS medium supplemented with 0.5 mg/L 6-BA,0.5 mg/L indole-3-butyric acid(IBA),30 g/L sucrose,and 7.5 g/L agar.The optimal rooting medium was half-strength MS medium supplemented with 0.5
作者
林妃
康勇
MUHAMMAD Dawood
周晨冉
李亚梅
郭玉华
尹俊梅
LIN Fei;KANG Yong;MUHAMMAD Dawood;ZHOU Chenran;LI Yamei;GUO Yuhua;YIN Junmei(Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Re-sources and Germplasm Enhancement in Southern China,Ministry of Agriculture and Rual Affairs/The Engineering Technology Re-search Center of Tropical Ornamental Plant Germplasm Innovation and Utilization,Haikou,Hainan 571101,China;Haikou Experimental Station,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;Department of Environmental Sciences,Bahauddin Zakariya University,Multan 60800,Pakistan;Yunnan Agricultural University,Puer,Yunnan 665000,China;Sanya Research Institute,Chinese Academy of Tropical Agricultural Sciences,Sanya,Hainan 572024,China)
出处
《热带作物学报》
CSCD
北大核心
2024年第6期1167-1174,共8页
Chinese Journal of Tropical Crops
基金
海南省重大科技计划项目(No.ZDKJ2021018)
海南省重点研发计划项目(No.ZDYF2023XDNY029)。
关键词
木棉
组织培养
消毒
诱导
生根
Bombax ceiba
tissue culture
disinfection
induction
rooting
