摘要
试验旨在提高枯草芽孢杆菌B. amyloliquefaciens T12甘露聚糖酶产酶量,验证水解产物的益生元活性。试验对发酵条件进行优化,单因素试验分别考察了8组培养变量对产酶活性影响,从影响β-甘露聚糖酶酶活力的单因素条件中筛选出3个主要因素,最陡爬坡试验确定主要因素中心点,最后采用Box-Behnken法设计并进行三因素三水平试验,通过Desgin Expert结果分析与后续试验验证。结果显示,最优发酵条件为魔芋粉浓度10.4g/L、酵母提取物6.37 g/L、装液量100 mL、接种量2%(体积分数)、发酵周期12~16 h、初始pH值6.5,其余条件不变。通过优化工艺发酵产酶,酶活力达到15 264.92 U/mL。对β-甘露聚糖酶水解产物甘露寡糖进行益生元活性分析,发现甘露寡糖对乳酸菌、干酪乳杆菌的生长均具有促进作用,且益生菌培养液具有抑制病原菌繁殖能力。
The purpose of the experiment was to increase the production of mannanase from B.amyloliquefaciens T12 and verify the prebiotic activity of the hydrolysate.The fermentation conditions were optimized,and the effects of eight groups of culture variables on the enzyme activity were investigated by single factor test.Three main factors were screened out from the single factor conditions affecting the enzyme activity ofβ-mannanase,and the center point of the main factors was determined by the steepest climbing test.Finally,Box-Behnken method was used to design and carry out the three factors and three levels test,which was verified by Desgin Expert results analysis and subsequent experiments.The results showed that the optimum fermentation conditions were as follows:Konjac powder concentration 10.4 g/L,yeast extract 6.37 g/L,liquid volume 100 mL,inoculation volume 2%(volume fraction),fermentation period 12~16 h,initial pH 6.5,and other conditions unchanged.The enzyme activity reached 15264.92 U/mL.The probiotic activity of mannooligosaccharides hydrolyzed byβ-mannanase was analyzed.It was found that mannooligosaccharides could promote the growth of Lactobacillus and Lactobacillus casei,and the probiotic medium could inhibit the proliferation of pathogenic bacteria.
作者
李传峥
白龙
高畅
崔岱宗
赵敏
Li Chuanzheng;Bai Long;Gao Chang;Cui Daizong;Zhao Min(Key Laboratory of Enzymes and Enzyme like Engineering in Heilongjiang Province,College of Life Sciences,Northeast Forestry University,Heilongjiang Harbin 150040;Research Center of Soil and Water Environment,Inner Mongolia University of Science and Technology,Inner Mongolia Baotou 014000)
出处
《现代畜牧兽医》
2024年第5期23-30,共8页
Modern Journal of Animal Husbandry and Veterinary Medicine
基金
国家自然科学基金面上项目(51678120)
中央高校基本科研业务费专项资金(2572020DY03)。
关键词
Β-甘露聚糖酶
枯草芽孢杆菌
发酵优化
益生元活性
甘露寡糖
β-mannanase
B.amyloliquefaciens
Fermentation optimization
Prebiotic activity
Mannan oligosaccharide
