摘要
目的 评估DNV-1929在犬肺中的强化效应和可操作性,通过观察其在手术切除过程中的表征为其临床应用提供科学依据。方法 15只10~15个月龄的比格犬,随机分组,植入DNV-1929和阴性对照材料。用特定的手术技术将材料植入犬的左肺前叶后部、后叶前部和后叶后部共3个部位,通过气道压力测试评估其耐压性能,并评估植入材料的操作性能。在植入后的第2周和第4周,对犬左肺3个植入材料部位进行宏观肉眼观察和病理检查,比较DNV-1929与阴性对照材料的组织反应。此外,观察所有实验动物临床症状、进食量和体质量变化以评估材料植入的安全性。结果 在气道压力水平高达35 hPa时,DNV-1929和阴性对照材料在植入部位均无气体泄漏,表现出相似的压力抗性。在手术过程中,DNV-1929显示出与阴性对照材料相似的可操作性,未观察到明显的异常情况。植入2周和4周后通过肉眼病理观察可见,两种材料均在植入部位周围形成包囊,并与胸腔器官发生不同程度的粘连,但差异无统计学意义(P>0.05)。植入DNV-1929组在2周时有较多的残留植入物,而植入4周时几乎消失,而阴性对照组还存在少量植入材料,但两组残留材料差异无统计学意义(P>0.05)。组织学评估结果显示,植入DNV-1929于2周时,炎症情况小计评分略高于阴性对照组(P=0.016),而植入4周的组间差异无统计学意义(P>0.05)。研究期间实验犬未出现临床异常或进食量减少,DNV-1929和阴性对照物当天无死亡。实验犬在研究周期内体质量无明显变化(P>0.05)。结论 DNV-1929在犬肺中表现出强化效应和良好的可操作性,且对周围组织无毒性影响。这为DNV-1929在肺部手术治疗中的应用提供了初步证据,为其在临床上的应用奠定了科学基础和其潜在价值。
Objective To assess the reinforcing effect and operability of DNV-1929 in canine lungs so as to provide scientific basis for its clinical application by observing its characterisation during surgical resection.Methods Fifteen Beagles of 10-to 15-month-old,randomly assigned to three experimental groups,were used in this study for implantation experiments with DNV-1929 and negative control material.The experimental animals were housed in a defined environment and detailed clinical observations were made before and after the experiments.By using specific surgical techniques,the materials were implanted into different parts of the left lung of the dogs and their pressure resistance was assessed by airway pressure tests.The handling properties of the implanted materials were also evaluated.At weeks 2 and 4 after implantation,macroscopic visual observations and pathological examinations were performed on three sites of the canine left lung,the posterior anterior anterior lobe,the anterior posterior lobe,and and the posterior posterior lobe,in order to compare the tissue response of DNV-1929 with that of the negative control material.In addition,all experimental animals were evaluated for safety,including monitoring of clinical signs,food intake,and body weight.Results At airway pressure levels up to 35 hPa,both DNV-1929 and the negative control material showed similar pressure resistance with no gas leakage at the implantation site.During the procedure,DNV-1929 showed similar manoeuvrability to the negative control material,with no significant abnormalities observed.As seen by visual pathological observation after 2 and 4 weeks of implantation,both materials formed an encapsulation around the implantation site and underwent different degrees of adhesion with the thoracic organs,but the difference was not statistically significant(P>0.05).There were more residual implants in the DNV-1929 group at 2 weeks and almost disappeared at 4 weeks of implantation,whereas a small amount of implanted material was still present in t
作者
老奋坚
关艺琳
松谷哲行
夏宇晓
张风华
LAO Fenjian;GUAN Yilin;SONGGU Zhexing;XIA Yuxiao;ZHANG Fenghua(Department of Respiratory Medicine,The Second Affiliated Hospital of Guangzhou Medical University,Panyu Campus,Guangzhou 510000,China;Department of Laboratory Medicine,The Fifth Affiliated Hospital of Guangzhou Medical University,Guangzhou 510000,China;Department of Thoracic Surgery,Gokokuji Hospital,Teikyo University School of Medicine,Tokyo 213-8507,Japan;Department of General Surgery,The Second Affiliated Hospital of Guangzhou Medical University,Panyu Campus,Guangzhou 510000,China)
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2024年第4期582-589,共8页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
广东省医学科研基金项目(No.A2023475)。
