摘要
目的探讨脓毒症患儿血清可溶性髓样细胞触发受体2(sTREM2)和转录因子阴阳-1(YY1)水平及其与心肌损伤的相关性。方法选取2020年10月至2023年10月期间于本院就诊的134例脓毒症患儿作为研究对象,根据患儿是否存在心肌损伤将其分为心肌损伤组(n=48)和无心肌损伤组(n=86);另选取同期健康儿童72例作为对照组。采用ELISA法检测各组血清sTREM2水平,采用qRT-PCR法检测各组血清YY1表达水平;Pearson法分析患儿血清sTREM2与YY1水平的相关性及二者血清水平与心肌损伤指标的相关性;多因素Logistic回归分析脓毒症患儿发生心肌损伤的影响因素。结果脓毒症患儿血清sTREM2和YY1水平均明显升高(P<0.05);无心肌损伤组和心肌损伤组患儿脓毒性休克占比、CRP、PCT、LD、CK-MB、cTnI、Mb水平比较,差异具有统计学意义(P<0.05);与无心肌损伤组相比,心肌损伤组患儿血清sTREM2和YY1水平明显降低(P<0.05),且血清sTREM2和YY1水平呈正相关(r=0.472,P=0.001),两者与心肌损伤指标LD、CK-MB、cTnI、Mb水平均呈负相关(均P<0.05);Logistic回归分析结果显示,脓毒症休克、CRP、PCT水平升高为脓毒症患儿发生心肌损伤的危险因素,sTREM2和YY1水平升高为保护因素(P<0.05)。结论脓毒症患儿血清sTREM2和YY1水平均明显升高,二者血清水平变化水平与脓毒症患儿心肌损伤密切相关,且sTREM2水平升高和YY1高表达为脓毒症患儿发生心肌损伤的保护因素。
Objective To investigate levels of serum soluble triggering receptor expressed on myeloid cells-2(sTREM2)and transcription factor Yin-Yang-1(YY1)in children with sepsis and their correlations with myocardial injury.Methods From October,2020 to October,2023,134 children with sepsis who visited our hospital were regarded as the study subjects.They were further divided into a myocardial injury group(n=48)and a non-myocardial injury group(n=86)based on whether they had myocardial injury;72 healthy children during the same period were regarded as the control group.ELISA method was applied to detect serum sTREM2 level in each group,and qRT-PCR method was applied to detect the serum YY1 expression level in each group;Pearson method was used to evaluate the correlation between serum sTREM2 and YY1 levels in pediatric patients,and the correlation between their serum levels and myocardial injury indicators;Multivariate logistic regression was applied to identify the influencing factors of myocardial injury in children with sepsis.Results Serum levels of sTREM2 and YY1 in children with sepsis were significantly increased(P<0.05);There was a significantly difference in the proportion of septic shock,levels of CRP,PCT,LD,CK-MB,cTnI,and Mb between the non-myocardial injury group and the myocardial injury group(P<0.05);Compared with the non-myocardial injury group,serum levels of sTREM2 and YY1 in myocardial injury group were significantly reduced(P<0.05),and serum levels of sTREM2 and YY1 were positively correlated(r=0.472,P=0.001),and both were negatively correlated with levels of myocardial injury indicators LD,CK-MB,cTnI,and Mb(all P<0.05);Logistic regression analysis showed that septic shock,elevated levels of CRP and PCT were risk factors for myocardial injury in children with sepsis,while elevated levels of sTREM2 and YY1 were protective factors(P<0.05).Conclusion Levels of serum sTREM2 and YY1 in children with sepsis are significantly increased,and their levels are closely related to myocardial injury in children with s
作者
张荣
杜健
杜超
ZHANG Rong;DU Jian;DU Chao(Department of Pediatric Intensive Care Medicine,Shanxi Children’s Hospital,Taiyuan 030025,China)
出处
《标记免疫分析与临床》
CAS
2024年第5期834-839,共6页
Labeled Immunoassays and Clinical Medicine
基金
山西省儿童医院与山西省妇幼保健院科研课题(编号:2021050)。
