摘要
为探究施用枯草芽孢杆菌对土壤真菌群落结构和作物生长的调控作用,本研究采用高通量测序技术分析枯草芽孢杆菌对草莓根际土壤真菌群落多样性和群落结构的影响,通过田间小区试验测定其对草莓根腐病的防治效果和对草莓的促生长作用。结果表明,施用枯草芽孢杆菌显著提高草莓根际土壤真菌多样性,Shannon指数、Ace指数和Chao指数显著上升,Simpson指数显著下降;门水平上子囊菌门(Ascomycota)相对丰度明显增加,担子菌门(Basidiomycota)和被孢菌门(Mortierellomycota)相对丰度明显减少;属水平上优势菌属毛壳菌属(Chaetomium)相对丰度明显增加,被孢霉属(Mortierella)、Tausonia和织球壳菌属(Plectosphaerella)相对丰度明显降低;枯草芽孢杆菌能有效降低草莓根腐病的发病率,定植2个月内的防治效果在70%以上,并显著提高草莓冠径、叶面积及叶绿素含量等生长指标值。综上,枯草芽孢杆菌能够增加草莓根际土壤中有益菌相对丰度,减少致病菌相对丰度,优化土壤真菌群落结构,有效防治草莓根腐病,促进草莓健康生长。
In order to explore the regulation of Bacillus subtilis on soil fungal community structure and crop growth,high⁃throughput sequencing technology was used to analyze the effects of B.subtilis on fungal di⁃versity and community structure in strawberry rhizosphere soil,and the effects on controlling strawberry root rot and promoting strawberry growth were determined by field plot experiment.The results showed that the applica⁃tion of B.subtilis significantly increased the fungal diversity in strawberry rhizosphere soil.The Shannon index,Ace index and Chao index increased significantly,and the Simpson index decreased significantly.At phylum level,the relative abundance of Ascomycota increased significantly,while that of Basidiomycota and Mortierel⁃lomycota decreased significantly.At genus level,the relative abundance of Chaetomium increased significant⁃ly,while that of Mortierella,Tausonia and Plectosphaerella decreased significantly.B.subtilis could effectively reduce the incidence of strawberry root rot with the control effect higher than 70%within 2 months of planting,and significantly increased the growth indexes such as canopy diameter,leaf area and chlorophyll content.Comprehensive analysis showed that B.subtilis could increase the relative abundance of beneficial fungi but re⁃duce that of pathogenic fungi in strawberry rhizosphere soil,so optimized the soil fungal community structure,effectively controlled strawberry root rot and promoted the healthy growth of strawberry.
作者
李宝庆
石亚冰
凌凡舒
徐丹
孙卿
刘玉珊
韩庆典
Li Baoqing;Shi Yabing;Ling Fanshu;Xu Dan;Sun Qing;Liu Yushan;Han Qingdian(Linyi Agricultural Technology Promotion Center,Linyi 276000,China;College of Agriculture and Forestry,Linyi University,Linyi 276000,China;Shandong(Linyi)Institute of Modern Agriculture,Zhejiang University,Linyi 276000,China)
出处
《山东农业科学》
北大核心
2024年第6期81-87,共7页
Shandong Agricultural Sciences
基金
山东省生态农业产业技术体系项目(SDAIT-30-12)
山东省农业重大应用技术创新项目(2015NY212)
浙江大学山东(临沂)现代农业研究院服务地方经济发展项目(ZDNY-2021-FWLY02004)
临沂大学社会服务项目(HX230195)。
关键词
枯草芽孢杆菌
草莓
高通量测序
真菌群落结构
草莓根腐病
Bacillus subtilis
Strawberry
High⁃throughput sequencing
Fungi community structure
Strawberry root rot
