摘要
目的 研究再生障碍性贫血患者外周血B淋巴细胞诱导成熟蛋白-1(Blimp-1)表达与调节性T细胞(Treg)/辅助性T细胞17(Th17)失衡及预后的关系。方法 选择2020年3月至2023年3月陕西中医药大学附属医院收治的140例再生障碍性贫血患者作为观察组,以同期体检的110名健康志愿者作为对照组。检测两组外周血Blimp-1表达水平、Treg及Th17细胞数目,血清白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、白细胞介素-17(IL-17)水平;评价观察组患者病情并分为重型患者和轻型患者,比较两组间外周血Blimp-1、Treg、Th17及血清IL-6、IL-10、IL-17的差异,采用Pearson检验分析外周血Blimp-1与Treg、Th17、IL-6、IL-10、IL-17的相关性;随访预后并计算预后不良发生率,采用K-M曲线比较两组间预后不良发生率的差异。结果 观察组外周血Blimp-1表达水平、Treg数目、Treg/Th17比值以及血清IL-10水平低于对照组,Th17数目、血清IL-6及IL-17水平高于对照组,差异有统计学意义(P<0.05);观察组外周血Blimp-1表达水平与Treg数目、Treg/Th17比值、IL-10水平呈正相关(r=0.351、0.376、0.328,P<0.05),与Th17数目及IL-6、IL-17水平呈负相关(r=-0.338、-0.409,P<0.05);观察组中重型患者的外周血Blimp-1表达水平、Treg数目、Treg/Th17比值以及血清IL-10水平低于轻型患者,Th17数目、血清IL-6及IL-17水平高于轻型患者,差异有统计学意义(P<0.05);观察组中Blimp-1高表达患者的预后不良发生率低于Blimp-1低表达患者,差异有统计学意义(P<0.05)。结论 外周血Blimp-1表达降低可能导致再生障碍性贫血患者Treg/Th17失衡、病情加重及预后不良。
Objective To investigate the relationship between the expression of B-lymphocyte in-duced maturation protein-1(Blimp-1)in peripheral blood and the imbalance of T regulatory cells(Treg)/T helper cell 17(Th17)and prognosis of aplastic anemia patients.Methods 140 patients diagnosed with aplastic anemia at the Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine from March 2020 to March 2023 were selected as the observation group,and 110 healthy volunteers who underwent physical exami-nations during the same period were selected as the control group.The expression level of Blimp-1 in peripheral blood,the quantities of Treg and Th17 cells,and the levels of interleukin-6(IL-6),interleukin-10(IL-10)and interleukin-17(IL-17)in serum were measured.The disease condition of the observation group was evaluated and divided into severe and mild cases.T tests were used to compare the difference of Blimp-1,Treg,Th17,in peripheral blood and IL-6,IL-10,IL-17 in serum between the two groups.Pearson test was used to analyze the correlation between Blimp-1 in peripheral blood and Treg,Th17,IL-6,IL-10,IL-17.The prognosis of aplastic anemia patients was followed up and the incidence of poor prognosis was calculated.K-M curve was used to compare the incidence of adverse prognoses between the two groups.Results The expression level of Blimp-1 in peripheral blood,Treg number,Treg/Th17 ratio and serum IL-10 level in the observation group were lower than those in the control group,and the Th17 number,serum IL-6 and IL-17 levels were higher in the observa-tion group compared to the control group,the difference was statistically significant(P<0.05).The expression level of Blimp-1 in peripheral blood of patients in the observation group was positively correlated with Treg num-ber,Treg/Th17 ratio and IL-10 level(r=0.351,0.376,0.328,P<0.05),and negatively correlated with Th17 number and IL-6 and IL-17 levels(r=-0.338,-0.409,P<0.05).The expression level of Blimp-1 in peripheral blood,Treg number,Treg/Th17 ratio and serum IL-1
作者
吴安琪
宁小康
张立
WU Anqi;NING Xiaokang;ZHANG Li(Practice Teaching Center of Shaanxi Energy Vocational and Technical College,Xianyang,Shaanxi,China,712000;Department of Blood Purification,Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Xianyang,Shaanxi,China,712000)
出处
《分子诊断与治疗杂志》
2024年第6期1138-1142,共5页
Journal of Molecular Diagnostics and Therapy
基金
湖北省卫生健康科研基金资助(WJ2019M203)。
