摘要
目的观察叉形头转录因子O3a(forkhead box O transcription factor 3a,FOXO3a)在子宫内膜样癌中的表达特征,分析其在不同临床及病理特征中的表达差异,探讨其对预后的判断价值。方法选取2016年1月至2018年5月期间在华北理工大学附属医院确诊为子宫内膜样癌并行手术根治的患者48例作为观察组,选取同期确诊为子宫内膜单纯性增生的子宫内膜组织32例作为对照组,选取同期确诊为增生期的子宫内膜组织22例作为正常对照组,应用免疫组化EnVision法检测三组中FOXO3a的蛋白表达水平。FOXO3a与术后生存时间的关系应用Kaplan-meier进行生存分析;应用The Cancer Genome Atlas(TCGA)数据库进行生信分析,观察FOXO3a mRNA在子宫内膜样癌组织和正常组织中FOXO3a mRNA表达的差异及其与临床病理特征的相关性;应用UCSC XENA工具分析FOXO3a mRNA表达水平与总体生存率的相关性。结果FOXO3a在子宫内膜样癌组织中表达的阳性率明显低于子宫内膜单纯性增生和增生期子宫内膜组织(FOXO3a在三组中的阳性率分别为14.58%、75%和95.5%),差异有统计学意义(P=0.000);FOXO3a在子宫内膜样癌不同年龄(P=0.311)、分化程度(P=0.730)、有无癌结节/淋巴结转移(P=0.902)、不同浸润深度(P=0.919)、增殖指数(P=0.165)及是否存在错配修复缺陷(P=1.000)的分组中差异无统计学意义(P>0.05);生存分析显示FOXO3a蛋白表达量与子宫内膜样癌术后总体生存时间无相关性(χ^(2)=0.2526,P=0.6152)。生信分析显示子宫内膜癌组织中FOXO3a mRNA的表达具有降低趋势(P<0.01),并与年龄显著相关(P=0.044),而与组织学分级、临床分期、总体生存期均无明显相关性(P>0.05)。结论FOXO3a在子宫内膜样癌组织中的表达下调,参与肿瘤的形成;子宫内膜样癌中FOXO3a的表达水平与患者的预后无明显相关性。
Objective To investigate the expression characteristics of Forkhead box O transcription factor 3a(FOXO3a)in endometrioid carcinoma,analyze its expression differences in different clinical and pathological features,and explore its value in predicting prognosis.Methods A total of 48 patients diagnosed with endometrioid carcinoma who underwent surgical resection at the Affiliated Hospital of North China University of Science and Technology from January 2016 to May 2018 were selected as the observation group.Additionally,32 patients diagnosed with endometrial simple hyperplasia and 22 patients diagnosed with proliferative phase endometrium during the same period were selected as the control and normal control groups,respectively.The protein expression level of FOXO3a in the three groups was detected by immunohistochemistry(IHC).The relationship between FOXO3a expression and postoperative survival time was analyzed using Kaplan-Meier analysis.Moreover,The Cancer Genome Atlas(TCGA)database was used to perform bioinformatics analysis to observe the differences in FOXO3a mRNA expression between endometrioid carcinoma tissue and normal tissue and its correlation with clinical pathological features.The UCSC XENA tool was used to analyze the correlation between FOXO3a mRNA expression level and overall survival.Results The positive expression rate of FOXO3a in endometrioid carcinoma tissue was significantly lower than that in the endometrial simple hyperplasia and proliferative phase endometrial tissue groups(14.58%,75%,and 95.5%,respectively).The difference was statistically significant(P=0.000).FOXO3a expression in endometrioid carcinoma showed no significant differences in age,differentiation degree,presence of tumor nodules/lymph node metastasis,depth of invasion,proliferative index,or mismatch repair deficiency(all P>0.05).Survival analysis showed no correlation between FOXO3a protein expression level and overall survival time after surgery(χ^(2)=0.2526,P=0.6152).Bioinformatics analysis showed that FOXO3a mRNA expressi
作者
唐慧
刘时飞
熊艳杰
罗丹
董丽儒
宋旭东
TANG Hui;LIU Shi-fei;XIONG Yan-jie;LUO Dan;DONG Li-ru;SONG Xu-dong(Department of Pathology,North China University of Science and Technology Affiliated Hospital,Tangshan 063000,Hebei,China;不详)
出处
《广东医学》
CAS
2024年第5期540-544,共5页
Guangdong Medical Journal
基金
河北省医学科学研究课题计划(20191135)。
