摘要
目的探讨快速超声组织处理系统对乳腺穿刺组织制片及切片染色效果的影响。方法随机选取2023年中国医科大学附属盛京医院病理科的乳腺穿刺标本100例,采用快速超声组织处理技术进行脱水处理(快速组),并对其相应的乳腺切除手术标本做0.3 cm×0.3 cm×0.3 cm取材及常规病理组织处理(常规组),所有病例术前未经新辅助治疗。2组标本脱水完成后均行常规切片。比较2组标本的脱水时间、HE染色效果、荧光原位杂交(FISH)检测结果,采用配对计数资料的Kappa检验比较2组雌激素受体(ER)、孕激素受体(PR)、人表皮生长因子受体2(HER2)免疫组织化学染色结果的一致性。结果快速组脱水时间[(46.70±1.42)min]明显短于常规组[(659.25±6.03)min],2组比较差异有统计学意义(t=987.44,P<0.001);快速组HE染色评分(10.13±1.12)与常规组染色评分(10.15±0.97)比较无统计学差异;免疫组织化学染色结果显示,2组ER表达一致率为92.5%(Kappa=0.794,P<0.05),PR表达一致率为76.25%(Kappa=0.639,P<0.05),HER2表达一致率为72.5%(Kappa=0.610,P<0.05);2组FISH检测结果一致。结论快速超声组织处理具有时间短、污染小、操作简单等优点,其组织切片效果与常规脱水比较无显著差异,值得在小的病理组织标本处理中推广应用。
Objective To investigate the effect of rapid ultrasonic tissue processing on breast biopsy tissue.Methods Totally 100 breast puncture samples from the Department of Pathology at Shengjing Hospital of China Medical University in 2023 were randomly selected and dehydrated using rapid ultrasound tissue processing technology(the rapid group).Paired surgical samples(0.3 cm×0.3 cm×0.3 cm)that did not undergo neoadjuvant therapy before surgery were collected and subjected to routine pathological tissue treatment(the conventional group).The two groups of samples were subjected to routine sectioning after dehydration.The dehydration time,hematoxylin and eosin(HE)staining effect,and fluorescence in situ hybridization(FISH)results of the two groups were compared.Next,the estrogen receptor(ER),progesterone receptor(PR),and human epidermal growth factor receptor 2(HER2)immunohistochemical(IHC)detection results of the two groups were compared using the kappa test with paired counting data.Results Dehydration time in the rapid group(46.70±1.42 min)was significantly shorter than that in the conventional group(659.25±6.03 min,t=987.44,P<0.001).No significant differences were observed between the HE staining scores of the rapid group(10.13±1.12)and that of the conventional group(10.15±0.97).The consistency rate of ER expression between the two groups was 92.5%(Kappa=0.794,P<0.05),that of PR expression between the two groups was 76.25%(Kappa=0.639,P<0.05),and that of HER2 expression between the two groups was 72.5%(Kappa=0.610,P<0.05).The FISH results were consistent between the two groups.Conclusion Rapid ultrasonic tissue treatment has the advantages of a short procedure time,less pollution,and simple operation.The present study showed no statistically significant differences between the effect of rapid tissue treatment on tissue sections and that of routine dehydration.Therefore,this method can be widely used in pathology for the analysis of small samples.
作者
王頔
王蕊
马铁
王哲
吕庆杰
WANG Di;WANG Rui;MA Tie;WANG Zhe;LÜQingjie(Department of Pathology,Shengjing Hospital of China Medical University,Shenyang 110004,China)
出处
《中国医科大学学报》
CAS
北大核心
2024年第6期481-486,共6页
Journal of China Medical University
基金
国家自然科学基金(82072095)。
