摘要
为研究E93在拟穴青蟹中的功能和调控特征,利用转录组测序和分子克隆鉴定获得了拟穴青蟹(Scylla paramamosain)的E93基因,命名为Sp-E93,并对其基本生物信息、系统进化、组织分布以及激素调控的模式进行了分析。结果发现,Sp-E93的mRNA长度为3756 bp,包含2982 bp开放阅读框(ORF),编码993个氨基酸,预测蛋白质的相对分子质量为106.34 kDa,含有两个保守的helix-turn-helix(HTH)结构域,而HTH结构域外的其他区域序列在不同物种中一致度较低。系统进化分析发现,该基因的进化与物种进化基本一致。Sp-E93在拟穴青蟹成体的Y器官和大颚器中表达量最高,且在雄蟹中的表达显著高于雌蟹(P<0.05)。此外,甲基法尼酯和甲氧普林能显著上调雌蟹肝胰腺中E93基因的表达,但对卵巢中E93的表达无调控作用;20E可以调控肝胰腺E93基因的表达,但对卵巢中E93的表达无显著调控。结果表明,青蟹的E93基因在拟穴青蟹的激素信号中发挥作用,但调控模式较昆虫发生了一定进化,研究结果可为进一步研究甲壳动物E93的功能奠定基础。
Ecdysteroids(Ecds)and juvenile hormones(JHs)coordinate to regulate arthropods growth and development.In insects,E93 is a primary response gene to 20-hydroxyecdysone(20E),which mediates the 20E-induced autophagy and apoptotic signals to trigger metamorphosis during development,while JH inhibits E93 expression through JH signal to prevent precious metamorphosis.Crustacean has different development patterns and life cycles,especially during metamorphosis,whether E93 still plays a pivotal role in crustacean development still needs to be elucidated.Currently,rare information is known about crustacean E93.In this study,we identified E93 gene from Scylla paramamosian,named Sp-E93.Sp-E93 was located at the position 15396399 in the positive chain of pseudo-chromosome LG26,and its genomic structure contained six exons and five introns,with a total length of 100679 bp.The mRNA length of Sp-E93 was 3756 bp,containing a 2982 bp open reading frame(ORF)encoding 993 amino acids with a predicted protein molecular mass of 106.34 ku.Two conserved helix-turn-helix(HTH)domains were found in Sp-E93,while LLQHLL was lost.Results from multiple sequence alignment revealed that E93 amino acids sequences were less consistent across species except two HTH domain regions.The phylogenetic tree indicated that the evolution of E93 was basically the same with species evolution,but many clades had a low node support,indicating that E93 might undergo evolution after species differentiation.Furthermore,the expression of Sp-E93 in different tissues of male and female adult crabs revealed that Sp-E93 had the highest expression in Y organ(YO)and mandibular organ(MO),and relatively low expression in midgut and muscle in both females and males,indicating that the expression of Sp-E93 might have some relationship with biosynthesis and secretion of Ecd and methyl farnesoate(MF),which were synthesized in YO and MO respectively.Significantly higher expression of Sp-E93 was found in males than females,this might be due to the different levels of hormone tite
作者
宋诗颖
张凤英
王伟
刘志强
马凌波
赵明
SONG Shiying;ZHANG Fengying;WANG Wei;LIU Zhiqiang;MA Lingbo;ZHAO Ming(College of Aquatic and Life Sciences,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Oceanic and Polar Fisheries,Ministry of Agriculture and Rural Affairs,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China)
出处
《海洋渔业》
CSCD
北大核心
2024年第3期307-315,共9页
Marine Fisheries
基金
上海市青年科技英才扬帆计划(21YF1459700)
中国水产科学研究院创新团队(2020TD20)。
