摘要
目的研究内源性骨膜引导骨再生过程中信号通路富集及卷曲蛋白(FZD)家族动态表达。方法建立猪闭合肋骨膜引导下的骨再生模型,选择7个时间点采集骨膜和再生骨组织进行基因测序,分析差异基因筛选以及相关信号通路富集的情况。选取与骨再生过程中密切相关的FZD分子家族,检测并分析FZD各成员的动态表达变化及其表达相关性。结果实验组相较于对照组,共有7个基因在建模后1个月内的不同时间点持续存在差异表达。差异基因富集显示,早期主要存在免疫反应,后实现组织发展和重塑。FZD家族各成员在不同时间点的表达存在波动,大多数成员的表达高峰在术后3 d,而实验组的倍数改变高峰在术后1个月最为明显,FZD1和FZD2最高,且二者具有较高的相关性。结论本研究筛选出的骨膜引导性骨再生过程中的差异基因以及相关信号通路,为进一步的分子机制研究提供了理论依据,而FZD分子家族动态表达变化在该过程中发挥着重要作用,其具体参与的生物机制尚需进一步研究验证。
Objective To investigate the signaling pathway enrichment and dynamic expression of FZD family during endogenous periosteum-guided bone regeneration.Methods A porcine closed rib periosteum-guided bone regeneration model was established,and periosteum and regenerated bone tissues were collected at seven time points for gene sequencing,differential gene screening and enrichment analysis of their related signaling pathways.The FZD molecular family,which is closely related to the process of bone regeneration,was selected for focused analysis,and the dynamic expression changes of each member of FZD and the expression correlation between them were detected and analyzed.Results A total of seven genes were persistently differentially expressed at different time points within 1 month after surgery in the experimental group compared with the control group.Differential gene enrichment showed that there was mainly an immune response in the early stage,and tissue development and remodeling were achieved later.The expression of each member of the FZD family fluctuated at different time points,and the expression peak of most members was 3 days after surgery,while the fold change of the experimental group was more obvious than that of the control group at 1 month after surgery,and FZD1 and FZD2 were the highest,and there was a high correlation between them.Conclusion The differential genes in the process of periosteumguided bone regeneration and the related signaling pathways screened in this study provide a theoretical basis for further molecular mechanism studies,while the dynamic expression changes of the FZD molecular family play an important role in the process,and the specific biological mechanisms involved in the process need to be verified by further studies.
作者
陈晓雪
余宝富
王紫
吴其峰
李青峰
魏皎
CHEN Xiaoxue;YU Baofu;WANG Zi;WU Qifeng;LI Qingfeng;WEI Jiao(Department of Plastic and Reconstructive Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China)
出处
《组织工程与重建外科》
CAS
2024年第3期285-292,共8页
Journal of Tissue Engineering and Reconstructive Surgery
基金
国家自然科学基金(81871572,82302820,82372546)
上海市临床重点专科(shslczdzk00901)
上海市科学技术委员会临床医学研究中心项目(22MC1940300)。
关键词
大段骨缺损
骨膜
FZD家族
内源性引导骨再生
Large segmental bone defects
Periosteum
FZD family
Endogenous guided bone regeneration
