摘要
大田软海绵酸(OA)在海产品中分布广泛,对食品安全造成极大威胁。因OA具有高毒性、小尺寸和低分子量的特性,使得其抗体的获得面临诸多困难。核酸适体SELEX筛选技术可针对小分子获得高亲和性、单一适体序列,在贝类生物毒素的检测中具备极大优势。本文对基于OA适体构建的生物传感器,如比色传感器、荧光传感器、电化学传感器等在OA快速检测中的应用进行了综述,并总结归纳了该类传感器的性能和应用潜力,讨论了适体传感器未来的发展方向,以期更好地促进其开发和应用。
Okadaic acid(OA)is widely distributed in seafoods and is a remarkable threat to food safety.Owing to its high toxicity,small size,and low molecular weight,obtaining antibodies against OA is a complex and difficult task.The aptamer SELEX technology can be used to achieve high affinity and single aptamer sequences for small molecules.Accordingly,this technology has several advantages for the detection of shellfish biotoxins.This review highlights the applications of biosensors based on OA aptamers,such as colorimetric sensors,fluorescent sensors,electrochemical sensors,and so on,in the rapid detection of OA.A summary of the performance and application potential of these sensors is provided,and the development direction of aptamer sensors is discussed to better promote their development and application.
作者
陈盼
李迎
杨丽霞
谭建锡
CHEN Pan;LI Ying;YANG Lixia;TAN Jianxi(Changsha County Food and Drug Safety Testing Center,Hu'nan Changsha 410010,China;Changsha Customs Technology Center,Hu'nan Changsha 410004,China;Changsha Institute for Food and Drug Control,Hu'nan Changsha 410036,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2024年第3期355-359,共5页
Chinese Journal of Food Hygiene
基金
长沙市自然科学基金项目(kq2014181)
国家市场监督管理总局科技计划项目(2020MK074)。
