摘要
目的:探讨昆仙胶囊治疗创伤性骨化性肌炎大鼠的可能作用机制。方法:选取78只SD大鼠,按随机数字表法分为空白组(6只)、假手术组(24只)、对照组(24只)及药物组(24只),空白组直接处死后取血,假手术组剪开踝关节表皮后予以缝合,对照组和药物组建立创伤性骨化性肌炎模型,对照组予安慰剂灌胃,假手术组和药物组均予昆仙胶囊汤剂灌胃,在术后14 d、28 d、42 d、56 d各断颈处死6只大鼠,测量造模前后踝关节的肿胀厚度并计算肿胀率,用酶联免疫吸附试验(ELISA)检测炎性因子肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、C-反应蛋白(CRP)和骨成型蛋白-4(BMP4)、Smad5水平,取跟腱组织用免疫组化检测BMP4和Smad5的表达情况。结果:对照组和药物组术后14 d、28 d、42 d、56 d的踝关节肿胀率均高于假手术组(P<0.01)。药物组术后42 d、56 d的踝关节肿胀率均低于对照组(P<0.01)。对照组和药物组术后14 d、28 d、42 d、56 d的血清TNF-α水平均高于假手术组(P<0.01);药物组术后28 d、42 d、56 d的血清TNF-α水平均低于对照组(P<0.01)。对照组术后14 d、28 d、42 d、56 d的血清IL-6水平均高于假手术组(P<0.01);药物组术后14 d、28 d、42 d的血清IL-6水平均高于假手术组(P<0.01)。药物组术后14 d、28 d、42 d、56 d的血清IL-6水平均低于对照组(P<0.01)。对照组和药物组术后14 d、28 d、42 d、56 d的血清CRP水平均高于假手术组(P<0.01);药物组术后42 d、56 d的血清CRP水平均低于对照组(P<0.01)。对照组术后14 d、28 d、42 d、56 d的血清BMP4水平均高于假手术组(P<0.01),药物组术后14 d、28 d、42 d的血清BMP4水平均高于假手术组(P<0.01);药物组术后28 d、42 d、56 d的血清BMP4水平均低于对照组(P<0.01)。对照组术后14 d、28 d、42 d、56 d的血清Smad5水平均高于假手术组(P<0.01),药物组术后14 d、28 d、42 d的血清Smad5水平均高于假手术组(P<0.01)。药物组术后56 d�
Objective:To investigate the possible mechanism of Kunxian Capsules in treating rats with traumatic myositis ossificans.Methods:Seventy-eight SD rats were selected and divided into the blank group(6 rats),the sham operation group(24 rats),the control group(24 rats)and the medicine group(24 rats)according to the random number table method.Rats in the blank group were directly executed and blood was collected;rats in the sham operation group were sutured after their ankle epidermis being cut;rats in the control group and the medicine group were established models with traumatic myositis ossificans;rats in the control group were given placebo by gavage;rats in the sham operation group and the medicine group were given decoction of Kunxian Capsules by gavage.Six rats were respectively executed by neck dissection on day 14,day 28,day 42 and day 56 after operation.The swelling thickness of ankle joint was measured before and after modeling and the joint swelling rates were counted.The levels of serum inflammatory factors[tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),C-reactive protein(CRP)],bone morphogenetic protein-4(BMP4)and Smad5 were detected by Enzyme-Linked Immunosorbent Assay(ELISA),and the expressions of BMP4 and Smad5 in achilles tendon tissue was detected by immunohistochemistry.Results:The ankle swelling rates in the control group and the medicine group on day 14,day 28,day 42 and day 56 after operation were higher than those in the sham operation group(P<0.01).The ankle swelling rates in the medicine group on day 42 and day 56 after operation were lower than those in the control group(P<0.01).The serum TNF-αlevels in the control group and the medicine group were higher than those in the sham operation group on day 14,day 28,day 42 and day 56 after operation(P<0.01);the serum TNF-αlevels in the medicine group were lower than those in the control group on day 28,day 42 and day 56 after operation(P<0.01).The serum IL-6 levels in the control group were higher than those in the sham operation group on
作者
陈位
王晔恺
金丹雯
CHEN Wei;WANG Yekai;JIN Danwen(Zhoushan Hospital,Zhoushan Zhejiang 316021,China)
出处
《新中医》
CAS
2024年第10期164-170,共7页
New Chinese Medicine
基金
浙江省中医药科技计划项目(2020ZB274)
浙江省卫健委医药卫生科技计划项目(2020KY340)。
