摘要
目的本研究旨在了解本中心体外循环变温水箱是否有非结核分枝杆菌(NTM)污染。方法在含氯消毒剂消毒水箱后次日,留取本中心1号楼19个水箱的水样,进行NTM培养。对于水样分枝杆菌培养阳性的水箱,用无菌拭子分别涂抹水箱内胆生物膜和水箱出风口处,做NTM培养和鉴定,采用rpoβ基因和16S rRNA基因片段聚合酶链反应扩增测序,将测序结果进行基本局部比对搜索工具(BLAST)比对以确定菌种。结果4个水箱的水样检测NTM阳性,2个水箱在内胆和出风口都检测到奇美拉分枝杆菌,1个水箱仅在内胆区检测到奇美拉分枝杆菌,1个水箱在内胆区和出风口分别检测到戈登分支杆菌和奇美拉分枝杆菌。结论本中心体外循环变温水箱存在NTM污染,以奇美拉分枝杆菌为主。应通过加强变温水箱NTM监测、使用过滤水、定期清除生物膜、定期消毒、合理摆放水箱等方法降低NTM污染和感染风险。
Objective This study aimed to investigate the situation of non-tuberculous mycobacterium(NTM)contamination in heater-cooler unit(HCU)used in cardiopulmonary bypass.Methods Water samples were collected from the nineteen HCU in our hospital and sent for NTM culture.Furtherly,NTM-positvie heater-cooler devices were sampled in water tank biofilm and air outlet for NTM identification via sequencing two DNA regions in rpoβand 16S rRNA.Results Water samples from four HCU were tested NTM positive.In two HCUs,M.chimaera were detected in both the water tank biofilms and air outlets.In the third HCU,M.chimaera was detected only in water tank biofilm.In the last HCU,M.chimaera and M.gordonae were detected in water tank biofilm and air outlet,respectively.Conclusion Several HCUs in our hospital were contaminated with NTM(mostly with M.chimera).Measure should be taken to reduce the risks of NTM contamination and HCU-related NTM infection:frequent NTM monitoring,using filtered tap water,scheduled biofilm removal,optimal HCU disinfection protocol,and placing HCU’s exhaust flow away from the operating field.
作者
闫姝洁
陶随
郑伟
杨林
亢一舟
王梦珍
李明
薄致远
窦红涛
吉冰洋
Yan Shujie;Tao Sui;Zheng Wei;Yang Lin;Kang Yizhou;Wang Mengzhen;Li Ming;Bo Zhiyuan;Dou Hongtao;Ji Bingyang(Department of Cardiopulmonary Bypass,Fuwai Hospital,Chinese Academy of Medical Sciences,Beijing 100037,China;Department of Laboratory Medicine,Peking Union Medical College Hospital,Peking Union Medical College&Chinese Academy of Medical Sciences,Beijing 100730,China)
出处
《中国体外循环杂志》
2024年第3期232-236,共5页
Chinese Journal of Extracorporeal Circulation
关键词
变温水箱
奇美拉分枝杆菌
非结核分枝杆菌
院内感染
Heater-cooler unit
Mycobacterium chimaera
Non-tuberculous mycobacterium
Nosocomial infection
