摘要
旨在分析猪流行性腹泻病毒(PEDV)感染仔猪空肠组织的转录组差异。用PEDV毒株HB(G2b)感染3头2日龄仔猪,对感染后18 h仔猪空肠组织进行免疫组化、转录组测序和分析,最后采用荧光定量PCR(RT-qPCR)验证转录组结果。结果显示:仔猪感染PEDV 18 h后空肠绒毛明显脱落、变短,且PEDV主要分布于空肠肠绒毛表面;GO富集及KEGG富集分析的差异基因主要与免疫应答过程和炎症反应过程相关;富集在趋化因子受体相互作用、Toll受体信号、脂肪与糖代谢等。结果提示PEDV感染可激活宿主免疫反应及相关抗病毒信号通路,并且引起宿主的炎症风暴和营养代谢紊乱,为深入研究PEDV的致病机制奠定了基础。
The aim is to study the pathogenic mechanism of porcine epidemic diarrhea virus(PEDV)by transcriptome analysis of the jejunum of two-day-old piglets infected with PEDV.Three two-day-old piglets were infected with the PEDV HB strain(PEDV-HB),which belongs to the currently prevalent G2b subtype.At 18 h post-infection,jejunum tissues were collected from piglets for pathology,PEDV detection,RNA-seq and transcriptome analysis.Finally;the transcriptome results were verified by quantitative real-time PCR(RT-qPCR).The result showed that the jejunal villi of piglets infected PEDV-HB were significantly shed and shortened;and was mainly distributed on the surface of intestinal villi.GO and KEGG enrichment analysis showed that differentially expressed genes(DEGs)were mainly related to immune response and inflammatory response,and were enriched in several classical pathways,such as cytokine-cytokine receptor interaction,Toll-like receptor signaling pathway,fat and glucose absorption and metabolism.(Conclusion)PEDV infection of the jejunum of piglets activates the host immune response and associated antiviral signaling pathways and induces a cytokine storm as well as dysfunctions in fat and glucose absorption and metabolism.In all,this research can lay the foundation for the study of pathogenesis mechanism of PED.
作者
李栋梁
郑关民
李帅
朱洪森
吴超
LI Dongliang;ZHENG Guanmin;LI Shuai;ZHU Hongsen;WU Chao(College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450046,China;College of Animal Science and Technology,Henan Agricultural University,Zhengzhou 450046,China;Henan University of Chinese Medicine School of Medicine,Zhengzhou 450046,China;Zhengzhou Customs,Zhengzhou 450008,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2024年第6期2652-2661,共10页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(32202826)
河南省大学生创新创业(202210471045)
河南省青年基金(222300420216)。
关键词
猪流行性腹泻病毒
转录组
致病机制
porcine epidemic diarrhea virus
transcriptions
pathogenesis mechanisms
