摘要
目的建立经饮水染铅小鼠模型,探讨孕期、哺乳期染铅对小鼠母代和子代海马及皮层组织形态学及母代小鼠的学习记忆能力的影响。方法30只成年雌性C57BL/6J小鼠按体重随机分为正常对照组(对照组)和低、高剂量染铅(低、高剂量)组。其中对照组自由饮用超纯水,低、高剂量组分别饮用0.5和2 g/L醋酸铅1周后合笼繁殖仔鼠,并持续染毒至其子代4周龄。采用Morris水密宫检测母代小鼠的学习与记忆能力,苏木素-伊红(HE)染色观察母代小鼠及仔鼠的海马与前额皮层的组织形态学改变。结果与对照组比较,低和高剂量组母代小鼠逃避潜伏期及高剂量组母代小鼠游泳路程延长,而低和高剂量组母代小鼠穿台次数降低(P<0.05,χ^(2)=20.381)。对照母鼠海马各区及皮层细胞染色均匀,细胞膜清晰,胞核圆润,细胞形态结构相对完整且排列良好。低剂量组母代小鼠CA3区及仔鼠海马CA1、CA2、CA3及DG区出现不同程度的细胞核固缩、碎裂,细胞结构不清等组织病理学改变。高剂量染铅可致母代小鼠及其仔鼠CA1、CA2、CA3及DG区出现不同程度的细胞核固缩、深染、碎裂,细胞收缩,结构模糊,细胞排列杂乱等组织病理学改变。低、高剂量组母代小鼠及仔鼠前额皮层出现细胞核固缩、肿胀,核仁破碎,细胞结构模糊。其损伤的程度随着染毒剂量的升高而增加。结论饮水染铅可引起母代小鼠海马及皮层神经细胞损伤,进而损伤认知功能,同时生命早期染铅可致子代小鼠海马、皮层组织形态学发生异常改变,以致神经细胞受损。
Objective To establish a mice model of lead(Pb)exposure through drinking water and explore the effects of Pb exposure during pregnancy and lactation on the pathological morphology of hippocampal and cortical tissues in both maternal and offspring mice,as well as the learning memory abilities of maternal mice.Methods Thirty adult female C57BL/6J mice were randomly divided into a control group and low and high-dose Pb exposed groups based on body weight.The control group received purified water freely,while low and high-dose Pb-exposed(low and high-dose)groups received 0.5 g/L and 2 g/L Pb acetate in their drinking water respectively for one week before breeding and continuously poisoned until their offspring reached four weeks of age.The Morris water maze was used to evaluate the learning memory abilities of the maternal mice,and HE staining was conducted to observe the pathological morphology changes in the hippocampi and prefrontal cortex tissues of both maternal mice and offspring.Results Compared to the control group,escape latency of the maternal mice in the low and high-dose group,and swimming distances of the high-dose group were increased,while the platform crossings in both the low and high-dose groups were decreased(P<0.05,χ^(2)=20.381).The hippocampi and cortical tissues of the control maternal mice exhibited uniform staining,clear cell membranes,round nuclei,and relatively intact and well-arranged cell structures.The low-dose group showed varying degrees of nuclear condensation and fragmentation,as well as unclear cell structures in the CA3 region of the maternal and offspring mice′s hippocampus,as well as in CA1,CA2,CA3,and DG regions of the hippocampi of offspring mice.High-dose Pb exposure led to nuclear condensation,deep staining,fragmentation,cell contraction,blurred structures,and disorganized cell arrangement in CA1,CA2,CA3,and DG regions of hippocampi in both maternal and offspring mice.In the prefrontal cortex of the low and high-dose groups of maternal and offspring mice,there were signs o
作者
韦若昆
韦佩祁
袁海燕
易湘
姜岳明
区仕燕
李少军
WEI Ruo-kun;WEI Pei-qi;YUAN Hai-yan;YI Xiang;JIANG Yue-ming;OU Shi-yan;LI Shao-jun(Department of Toxicology,School of Public Health,Guangxi Key Laboratory of Environment and Health Research,and Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases,Guangxi Medical University,Nanning,Guangxi 530021,China)
出处
《毒理学杂志》
CAS
2024年第2期129-134,140,共7页
Journal of Toxicology
基金
国家自然科学基金(NSFC82160626,81803281)
广西自然科学基金(2018GXNSFBA050060)
广西自然科学基金创新科研团队(2019GXNSFGA245002)。
关键词
铅
小鼠
海马
病理形态学
Lead
Mice
Hippocampus
Pathological changes
