摘要
目的 探讨T2DM合并骨质疏松症(OP)患者外周血单个核细胞(PBMCs)的长链非编码RNA(lncRNA)H19表达及与T2DM合并OP进展的关系。方法 选取2022年12月至2023年6月于连云港市第一人民医院内分泌科治疗的T2DM患者176例,根据双能X线测量的骨密度(BMD)分为单纯T2DM组(n=76)和OP组(n=100)。比较两组一般资料及生化指标,Spearman相关分析lncRNAH19与其他指标的相关性,Logistic回归分析T2DM合并OP的影响因素,受试者工作特征(ROC)曲线分析lncRNAH19对T2DM合并OP的诊断价值,多元线性回归分析lncRNAH19的影响因素。结果 与T2DM组比较,OP组女性占比、年龄、HbA1c、TC、TGF-β1升高(P<0.05),BMI、SUA、BMD、lncRNAH19降低(P<0.05)。Spearman相关分析显示,lncRNAH19表达与BMI、SUA、BMD呈正相关(P<0.05),与年龄、HbA_(1)c、TGF-β1呈负相关(P<0.05)。Logistic回归分析显示,年龄、BMI、TC、TGF-β1、lncRNAH19是T2DM合并OP的影响因素。ROC曲线分析显示,lncRNAH19诊断T2DM合并OP的曲线下面积为0.839,敏感度为76.3%,特异度为86.0%。多元线性回归分析显示,年龄、TGF-β1及有无OP是lncRNAH19的影响因素。结论 T2DM合并OP患者PBMCs的lncRNAH19表达降低,通过糖代谢、lncRNAH19/TGF-β1通路参与T2DM合并OP的发生发展。
Objective Exploring the clinical application value of long chain non-coding RNA(lnc RNA) H19 in peripheral blood mononuclear cells(PBMCs) in type 2 diabetes mellitus(T2DM) patients with osteoporosis(OP).Methods A total of 176 patients with T2DM admitted to the Department of Endocrinology of the First People's Hospital of Lianyungang City from December 2022 to June 2023 were selected.They were divided into OP group(n=100) and simple T2DM group(n=76) according to the results of bone mineral density(BMD) determination by dual-energy X-ray.General data and biochemical indicators were compared between the two groups.The correlation between lncRNA H19and other clinical indicators was analyzed by Spearman correlation analysis and the influencing factors for T2DM with OP were analyzed by logistic regression.Receiver operating characteristic(ROC) curve was used to evaluate the diagnostic value of lncRNA H19 in T2DM with OP.Results Compared with T2DM group,the proportion of females,age,HbA_1c,TC and TGF-β1 were higher(P<0.05),while BMI,SUA,BMD and lnc RNA H19 were lower in OP group(P<0.05).Spearman correlation analysis showed that lnc RNA H19 expression level was positively correlated with BMI,SUA and BMD(P<0.05),and negatively correlated with age,Hb A1c and TGF-β1(P<0.05).Logistic regression analysis showed that age,BMI,TC,TGF-β1 and lncRNA H19 were the influencing factors for T2DM combined with OP(P<0.05).ROC curve analysis showed that the AUC of lncRNA H19 in the diagnosis of T2DM with OP was 0.839,the sensitivity was 76.3%,and the specificity was 86.0%.Multiple linear regression analysis showed that age,TGF-β1 and OP were the influencing factors for lncRNA H19(P<0.05).Conclusion Lnc RNA H19 expression decreased in PBMCs in patients with T2DM with OP,which may participate in the occurrence and development of T2DM with OP through glucose metabolism and lnc RNA H19/TGF-β1pathway.
作者
杨腾
王娜
冯秀丽
张桐毓
郑之陈
王国凤
YANG Teng;WANG Na;FENG Xiuli(Department of Endocrinology,Jinzhou Medical University Lianyungang First People's Hospital Graduate Training Base,Lianyungang 222000,China)
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2024年第5期364-368,共5页
Chinese Journal of Diabetes
基金
2022年江苏省老年健康科研项目(LKM2022065)
2021年连云港市老龄健康科研项目(L202105)
2022年连云港市卫生健康科技项目(202206)
南京医科大学康达学院2021年科研发展基金(KD2021KYJJZD087)
连云港市科协2023年度软课题研究项目(Lkxyb23130)
2023年连云港市第六期“521”工程科研资助立项项目(LYG06521202372)。
