摘要
为获得具有异养硝化-好氧反硝化能力的高效脱氮菌,从沈阳市南部污水厂活性污泥中筛选分离得到能高效脱氮的异养硝化-好氧反硝化菌株.通过筛选得到一株脱氮能力最强的菌株,命名为Paracoccus sp.QD-30.通过测定16SrRNA基因序列确定该菌株为副球菌属,并进一步研究了Paracoccus sp.QD-30菌株在不同氮源环境下的脱氮特性和单因素环境因子的最佳脱氮条件.菌株最大氨氮去除率为87.69%,最大亚硝态氮去除率为77.19%,最大硝态氮去除率为77.25%.最佳脱氮条件:琥珀酸钠为最佳碳源、C/N质量比为14、温度为35℃、pH为7,转速为140 r/min.菌株Paracoccus sp.QD-30是一株新型且具有高效脱氮能力的副球菌,在生物脱氮领域具有较好的工程应用前景.
To obtain efficient denitrifying bacteria with heterotrophic nitrification-aerobic denitrification capacity,a heterotrophic nitrifying-aerobic denitrifying strain with efficient denitrification was screened and isolated from the activated sludge of the southern wastewater treatment plant in Shenyang.A strain named Paracoccus sp.QD-30.was identified as Paracoccus sp.by 16SrRNA gene sequencing.The studied Paracoccus sp.nitrogen removal characteristics of strain QD-30 under different nitrogen sources and the best nitrogen removal conditions under single environmental factors.The maximum removal rates of ammonia nitrogen,nitrite nitrogen and nitrate nitrogen were 87.69%,77.19%and 77.25%,respectively.The optimum denitrification conditions were as follows:sodium succinate as the best carbon source,C/N of 14,temperature of 35℃,pH of 7 and rotational speed of 140 r/min.Paracoccus sp.QD-30 is a new type of paracoccus with high denitrification capacity,which has good engineering application prospects in the field of biological denitrification.
作者
王佳宝
张宇红
董先博
许家琪
WANG Jiabao;ZHANG Yuhong;DONG Xianbo;XU Jiaqi(Shenyang University of Chemical Technology,Shenyang 110142,China)
出处
《沈阳化工大学学报》
CAS
2023年第6期498-505,541,共9页
Journal of Shenyang University of Chemical Technology
基金
辽宁省教育厅面上项目(LJKZ0471)
辽宁省应用基础研究计划(2023JH2/101300047)。
关键词
异养硝化-好氧反硝化
生物脱氮
脱氮特性
副球菌
heterotrophic nitrification-aerobic denitrification
biological nitrogen removal
denitrification characteristics
paracoccus
