摘要
目的分析2021-2022年渝东北片区聚集性诺如病毒感染毒株遗传多样性,促进重点人群和场所诺如病毒感染防控。方法采集聚集性诺如病毒感染事件中患者肛拭子及环境拭子,实时荧光定量PCR(qRT-PCR)检测诺如病毒核酸,逆转录PCR扩增病毒RNA聚合酶/衣壳蛋白片段并测序分析,诺如病毒分型工具网站(http://www.rivm.nl/mpf/norovirus/typingtool)确定基因型,运用MEGA-X软件按最大似然法构建种系发生树。结果共调查渝东北片区4个区县11起聚集性诺如病毒感染事件,qRT-PCR检测55份样品,均为GII基因群诺如病毒,经测序存在6种基因型。GII.17[P17]基因型导致4起疫情,GII.17[P17]和GII.1[P16]基因型混合感染导致1起疫情,GII.6[P7]及GII.8[P8]基因型各引起2起疫情,GII.2[P16]及GII.3[P12]基因型分别导致1起疫情。种系发生分析显示55株GII基因群毒株分布在5个基因簇。不同事件相同基因型的诺如病毒都位于同一个基因簇。不同事件不同基因型的诺如病毒各自聚集成簇,分属于不同的基因簇。结论2021-2022年渝东北片区11起聚集性诺如病毒感染毒株涉及多种基因型,GII.17[17]是引起聚集性感染事件的常见基因型,绝大多数事件由单一传染源引起,不同事件相同基因型的诺如病毒存在遗传相关性。
Norovirus is the global leading cause of epidemic and endemic acute gastroenteritis in people of all ages.To investigate the genetic diversity of GII genogroup noroviruses linked to clustered infections in northeast Chongqing,we collected anal swabs or environmental smears from 11 norovirus outbreaks during 2021-2022.Norovirus RNA was detected by quantitative real-time PCR(qRT-PCR),and partial viral RdRp/capsid genes were amplified by reverse transcription PCR(RT-PCR) and sequenced.Among samples from 11 outbreaks in 4 districts and counties,55 strains of GII genogroup norovirus were detected.Six genotypes were identified with an online norovirus genotyping tool(http://www.rivm.nl/mpf/norovirus/typingtool).Genotype GII.17[P17] was associated with four outbreaks;the co-circulating GII.17[P17] and GII.1[P16] caused another outbreak;GII.6[P7] and GII.8[P8] respectively were linked to two outbreaks;and GII.3[P12] and GII.2[P16] respectively accounted for one outbreak.Phylogenetic analysis also indicated that 55 GII genogroup strains formed five clusters,with noroviruses of identical genotypes from diverse events belonging to the same cluster,and that genetically distinct genotypes from diverse events belonged to different clusters.Therefore,our results revealed that multiple genotypes associated with norovirus outbreaks were circulating in northeast Chongqing,and GII.17[P17] was the predominant genotype linked to these outbreaks during 2021-2022.Most norovirus outbreak events were caused by single sources,and genetic relationships were demonstrated among noroviruses of identical genotypes from diverse events.
作者
郎中凯
陈爱平
王恒芹
甘雨露
张拥军
LANG Zhong-kai;CHEN Ai-ping;WANG Heng-qin;GAN Yu-lu;ZHANG Yong-jun(Wanzhou Center for Disease Control and Prevention,Chongqing 404100,China;Chongqing Medical and Pharmaceutical College,Chongqing 401331,China;Chongqing Key Laboratory for High Pathogenic Microbes,Chongqing 400042,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2024年第5期448-453,共6页
Chinese Journal of Zoonoses
基金
重庆市教委科学技术研究项目(No.KJZD-K202202802)
重庆医药高等专科学校基金项目(No.YGZ2021107)。
