摘要
目的:探讨经方二妙散(EMS)对胶原诱导型关节炎(CIA)大鼠脾脏和关节中免疫调控基因Gnαq表达的影响。方法:建立CIA大鼠模型,并随机分为模型组(CIA组)、1.5、3.0和4.5 g/kg二妙散组(L-EMS、M-EMS和H-EMS组)、阳性对照组(MTX,氨甲蝶呤),各组给予相应药物进行治疗,同时设立健康对照组(Control组)与模型组给予等量生理盐水灌胃。ELISA技术检测各组炎症因子IL-1β、IL-6和TNF-α的表达,使用qRT-PCR和Western blot分析Gnαq mRNA和蛋白的表达,免疫组化技术对脾脏和关节中的Gnαq进行定位。结果:CIA组大鼠关节出现明显组织受损、组织增生与炎症浸润等特征改变;MTX组和EMS组关节组织破坏及增生减轻;CIA组IL-1β、IL-6和TNF-α的表达显著升高(P<0.01);MTX组和EMS组IL-1β(除H-EMS组)、IL-6和TNF-α的表达显著低于CIA组(P<0.05)。L-EMS组IL-1β、IL-6和TNF-α的表达显著低于M-EMS和H-EMS组(P<0.05,P<0.01)。CIA组大鼠脾脏和关节中Gnαq mRNA和蛋白表达显著高于Control组(P<0.01);EMS和MTX组脾脏和关节中Gnαq mRNA和蛋白表达显著低于CIA组(P<0.05);1.5 g/kg EMS组Gnαq mRNA和蛋白的表达量明显低于其他组(P<0.01,P<0.05)。不同实验组大鼠脾脏和关节中均检测到不同程度的阳性信号。结论:CIA大鼠关节和脾脏组织中Gnαq表达增强可能导致炎症反应,可能通过下调Gnαq减轻CIA大鼠炎症反应。
Objective:To explore the effect of different concentrations of Chinese herbal formula,Er-miao-san(EMS)immune regulatory gene on Gnαq expression in rats with collagen-induced arthritis(CIA).Methods:In the study,we established a CIA model,and randomly divided into model groups,EMS group with 1.5,3.0 and 4.5 g/kg(L-EMS,M-EMS,H-EMS)were adminis-tered to CIA rats by gavage,methotrexate was used as a positive control group(MTX).The rats in each group were given correspond-ing drugs for treatment,and the normal control group and model group were given the same amount of normal saline gavage.ELISA detects the expression of IL-1β,IL-6 and TNF-αin rats'serum.The mRNA and protein expression of Gnαq were analyzed using qRT-PCR and Western blot.The localization of Gnαq was performed by IHC.Results:The joint of rats in CIA group showed obvious damage,tissue hyperplasia and inflammatory infiltration;the joint tissue destruction and proliferation of MTX and EMS groups were reduced;the expressions of IL-1β,IL-6 and TNF-αin CIA group were extremely increased(P<0.01).The expressions of IL-1β(except H-EMS group),IL-6 and TNF-αin MTX and EMS groups were significantly lower than those in CIA group(P<0.05).The expressions of IL-1β,IL-6 and TNF-αin L-EMS group were lower than those in the M-EMS and H-EMS groups(P<0.05,P<0.01).The expression of Gnαq mRNA and protein in spleen and joints of CIA group were higher than those of Control group(P<0.01).The expression of Gnαq mRNA and protein in spleen and joints of EMS and MTX groups were lower than those of CIA group(P<0.05).The expression of Gnαq mRNA and protein in the dose of 1.5 g/kg of EMS group was lower than those of the other groups(P<0.01,P<0.05).Different degree of posi-tive signal was detected in spleen and joints in different groups.Conclusion:Gnαq is expressed in spleen and joints of CIA rats,may participate in the formation of inflammatory response in CIA rats,and then mediate the formation and development of RA by down-reg-ulating the expression of Gnαq.mRNA and
作者
芦文静
李振
郝慧琴
高玉亭
王泽
刘进
LU Wenjing;LI Zhen;HAO Huiqin;GAO Yuting;WANG Ze;LIU Jin(Basic Laboratory of Integrative Chinese and Western Medicine,Shanxi University of Traditional Chinese Medicine,Jinzhong 030619,China;School of Basic Medicine,Shanxi University of Traditional Chinese Medicine,Jinzhong 030619,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2024年第6期1222-1227,共6页
Chinese Journal of Immunology
基金
国家自然科学基金项目(81904034)
山西省自然科学基金面上项目(201801D121228)
山西中医药大学基础研究专项[2020PY-JC(Y)-07,2020PY-JC(Y)-08]
山西中医药大学博士启动基金项目(2020BK02)
山西中医药大学青年科学家培育专题资助项目(2021PY-QN-04)
山西中医药大学科技创新团队重点项目(2018TD-011)
山西中医药大学校级研究生创新项目(2020CX023)
山西省研究生教育创新项目(2019SY516)。
