摘要
口腔种植修复术失败的主要原因是术后细菌在种植体表面黏附形成生物膜并导致周围炎症,主要致病菌为牙龈卟啉单胞菌(P.gingivalis,P.g),植入物相关感染严重影响手术效果及增加患者痛苦与费用,因此须赋予植体表面抗菌能力以降低感染发生率。微弧氧化(Micro-arc oxidation,MAO)技术是通过高电压形成牢固结合且具备良好骨整合性能的氧化涂层,同时已有研究发现镁及其化合物(氧化镁)具有良好抗菌性和生物相容性。将MAO与电泳沉积(Electrophoretic deposition,EPD)技术结合,在多微孔的二氧化钛表面沉积纳米氧化镁(nano-MgO)涂层,并评价其体外抗菌性能及生物相容性。通过SEM、XRD、EDS观察样品表面形貌结构、测定元素组成。通过稀释涂板计数法、细菌活死染色及SEM观察评价nano-MgO涂层对P.g的体外抗菌性能。通过将人牙龈成纤维细胞(HGF)与nano-MgO涂层共培养后CCK-8法、细胞活死染色及骨架染色观察评价nano-MgO涂层体外生物相容性。研究结果发现,nano-MgO颗粒在二氧化钛多微孔表面均匀-团聚沉积且覆盖率随沉积时间增加。各组样品对P.g的体外抗菌性能在24 h为6%~54%,在72 h为39%~79%。显微观察(活死及SEM)样品表面活菌比例随沉积时间而减少。各组样品与HGF共培养1 d后细胞相对存活率为79%–67%,5 d后为93–85%。荧光显微观察发现MAO钛样品表面几乎无死细胞,其余4组表面死细胞比例随沉积时间增加,各组样品表面细胞形态完整且各组间无明显差异。MAO钛表面EPD nano-MgO涂层具备良好体外抗菌性能及生物相容性,研究成果可为降低口腔种植修复术的感染发生率、减少患者痛苦及手术费用提供一种新方法。
Owing to their mechanical properties and biosafety,titanium(Ti)implants are widely used to replace missing teeth;however,their non-antimicrobial properties can lead to infection.The main reasons underlying the failure of oral implant repair are the biofilms and surrounding inflammation caused by bacterial adhesion to the surface of the implants.Implant-related infections considerably influence the effect of surgery and increase the pain and cost incurred by patients;the main pathogen is Porphyromonas gingivalis(P.g).Therefore,endowing the surface of implants with antibacterial ability to reduce the adhesion and colonization of bacteria on the surface of implants,thus reducing the incidence of infection,is necessary.Microarc oxidation(MAO)is currently one of the primary technologies used for implant surface modification.It can form porous titanium dioxide coatings on Ti with strong adhesion under high voltages.Moreover,the introduction of elements(such as calcium and phosphorus.)can promote bone healing and improve the osseointegration properties of the implant.However,owing to the rough and porous surface of micro-arc oxidation Ti(MAO-Ti),bacteria can easily attach and reproduce;therefore,infection will still occur.Magnesium and its compound[magnesium oxide(MgO)]have been found to have excellent antibacterial ability and biocompatibility.Therefore,in this study,MAO and electrophoretic deposition(EPD)were combined to deposit nano-magnesium oxide(nano-MgO)coatings on MAO-Ti for 0,15,30,45,or 60 s,while maintaining its biosafety.The MAO-Ti surface was endowed with antibacterial properties to reduce the incidence of infection.In this study,the in vitro antibacterial properties and biocompatibility of the samples were evaluated,the surface morphology and element composition of the samples were observed by scanning electron microscope(SEM),X-ray diffractometer(XRD),and energy dispersive spectrometer(EDS),the in vitro antibacterial properties of the samples against P.g were evaluated by dilution plate counting,bacterial
作者
范鑫丽
杜佳恒
肖东琴
李耀华
胡丽群
贺葵
翁杰
段可
刘刚利
FAN Xinli;DU Jiaheng;XIAO Dongqin;LI Yaohua;HU Liqun;HE Kui;WENG Jie;DUAN Ke;LIU Gangli(Oral and Maxillofacial Surgery,Stomatological Hospital of Shandong University,Jinan 250012,China;Research Institute of Tissue Engineering and Stem Cells,Nanchong Central Hospital,Nanchong 637000,China;Bone and Joint Surgery,Southwest Medical University Affiliated Hospital,Luzhou 646000,China;School of Medicine,Southwest Jiaotong University,Chengdu 610031,China)
出处
《中国表面工程》
EI
CAS
CSCD
北大核心
2024年第2期211-219,共9页
China Surface Engineering
基金
国家自然科学基金(52071277)
四川省科技计划(2020YFS0455,2022YFS0628)
南充市市校合作项目(20SXQT0335,22SXJCQN0002)
泸州市-西南医科大学合作项目(2020LZXNYDZ08,2020LZXNYDF02)
西南医科大学产学研项目(2022CXY03)
泸县西南医科大学联合课题(2020LXXNYKD-01)。
关键词
口腔种植体
微弧氧化
电泳沉积
氧化镁
抗菌
dental implants
micro-arc oxidation
electrophoretic deposition
magnesium oxide
antibacterial
