摘要
该研究依托网络药理学预测分析黄芪甲苷(astragalosideⅣ)对调节慢性萎缩性胃炎的功效,为其在功能性食品中的应用提供依据。该实验从Swiss Target Prediction、PharmMapper、OMIM等数据库中获取黄芪甲苷调节慢性萎缩性胃炎的目标基因,取交集确定靶点基因。在STRING数据库上构建蛋白质相互作用(protein-protein interaction,PPI)网络。PPI网络依据介数中心性用Cytoscape软件进行拓扑分析,并通过Autodock Vina软件模拟可能的分子对接结果。在微生信在线平台进行GO和KEGG富集分析,筛选出相关信号通路。体外实验通过1-甲基-3-硝基-1-亚硝基胍(1-methyl-3-nitro-1-nitrosoguanidine,MNNG)诱导人胃黏膜上皮细胞并用黄芪甲苷干预,来检测细胞活力及炎症因子分泌水平进行验证。最终筛选得到黄芪甲苷调节慢性萎缩性胃炎的潜在靶点53个,其中关键基因8个。GO富集分析显示,囊泡腔是生物过程发生的主要场所。KEGG结果表明,肿瘤中的蛋白聚糖是黄芪甲苷调节慢性萎缩性胃炎的关键信号通路。体外实验发现,黄芪甲苷对MNNG诱导的细胞损伤具有预防治疗作用,且可以下调IL-6、IL-8、IL-1β、TNF-α等炎症因子的分泌(P<0.05)。该研究表明黄芪甲苷可以通过多个靶点和途径发挥抗慢性萎缩性胃炎作用,可作为功能因子用于防治慢性萎缩性胃炎。
This study used network pharmacology to forecast and evaluate effectiveness in controlling chronic atrophic gastritis,laying the groundwork for its inclusion in functional foods.The target genes of astragalosideⅣthat control chronic atrophic gastritis were gathered for this experiment from databases such as Swiss Target Prediction,PharmaMapper,OMIM,etc.The target gene was identified by taking intersections.Based on the STRING database,a protein-protein interaction(PPI)network was created.Based on the centrality of the intermediate number,the PPI network did topology analysis using Cytoscape software,and simulated potential molecular docking outcome with Autodock Vina software.GO and KEGG enrichment analysis was conducted on the bioinformatics online platform to screen out relevant signal pathways.In vitro experiments were conducted by inducing GES-1 cells(human gastric mucosal epithelial cells)with MNNG(1-methyl-3-nitro-1-nitrosoguanidine)and treating them with astragalosideⅣto detect cell viability and inflammatory factor secretion levels for validation.Finally,53 potential targets were identified for the regulation of chronic atrophic gastritis by astragalosideⅣ,including 8 key genes.GO enrichment analysis showed that the vesicular cavity was the main site for biological processes to occur.The KEGG results indicated that proteoglycans in tumors were a key signaling pathway for astragalosideⅣto regulate chronic atrophic gastritis.In vitro experiments found that astragalosideⅣhad a preventive and therapeutic effect on MNNG-induced cell damage,and could downregulate IL-6,IL-8,IL-1β,and TNF-α(P<0.05).This study showed that astragalosideⅣcould operate as a functional factor for the prevention and treatment of chronic atrophic gastritis and could exercise its anti-chronic atrophic gastritis impact through many targets and pathways.
作者
包哲隈
胡舒楠
杨兰珠
喻文娟
杨靖亚
BAO Zhewei;HU Shunan;YANG Lanzhu;YU Wenjuan;YANG Jingya(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;College of Life Science,Shanghai Ocean University,Shanghai 201306,China;Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation(Shanghai),Ministry of Agriculture,Shanghai 201306,China;National R&D Branch Center for Freshwater Aquatic Products Processing Technology(Shanghai),Shanghai 201306,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2024年第12期284-291,共8页
Food and Fermentation Industries
关键词
黄芪甲苷
慢性萎缩性胃炎
药食同源
网络药理学
分子对接
astragalosideⅣ
chronic atrophic gastritis
homologous
network pharmacology
molecular docking
