摘要
【目的】探究桃果皮叶绿素降解规律及叶绿素降解相关基因的表达情况,以期为桃果实成熟期及底色差异判定确定依据。【方法】以春美、2-50等不同桃品种/品系为研究对象,测量成熟前桃果实色差、叶绿素含量等指标,用荧光定量PCR检测叶绿素降解相关基因的表达情况,通过分析叶绿素与相关基因表达的关系,确定影响桃果皮叶绿素降解的关键因素。【结果】桃果实成熟前30 d叶绿素含量降低与PpCLH2、PpSGRL高表达有关。PpCLH1基因在桃果实转色时表达量升高。在果实成熟前8~0 d,果皮叶绿素降解,PpSGR基因表达量上调。PpSGR基因表达情况与春美、2-50、中桃7号的叶绿素含量呈显著负相关,是导致桃果实成熟时叶绿素降解的关键基因。【结论】成熟前12~8 d是桃果皮关键转色期,PpSGR可能是桃果实成熟前果皮叶绿素降解的关键基因,这对进一步解析桃果实发育过程中果皮叶绿素降解机制提供了一种新的思路,也为探索桃果实发育过程中叶绿素降解的分子机制提供参考。
【Objective】Peach(Prunus persica),is one of the most important deciduous fruit trees in the world.The visual appeal of peach fruits,particularly their color,is a critical factor influencing consumer purchasing decisions.Chlorophyll content is important in determining the skin color and overall appearance of peaches.The precise role of chlorophyll degradation genes within the peach genome needs to fully understand,and their potential effects on chlorophyll breakdown in the peach peel and fruit ripening remain unclear.This study aimed to lay preliminary groundwork for understanding the ripening period and the underlying color differences in peach fruits by examining the color changes in the peach peels during the process of chlorophyll degradation,and the expression patterns of the genes related to the chlorophyll degradation.【Methods】Four peach varieties including Chunmei and 2-50 were chosen for this investigation.The color variations in the peach fruits prior to ripening were quantified using a colorimeter,and chlorophyll content was measured with a UV spectrophotometer.Although the primary chlorophyll degradation pathway has been identified,there are only limited researches on gene expression in the fruits.To fill this gap,quantitative transcription analysis was conducted,and the coding sequences(CDS)of relevant genes were sourced from genomic databases.The expression of the chloro phyll degradation-associated genes was quantified using real-time quantitative polymerase chain reaction(qRT-PCR),and the correlation between the chlorophyll degradation and the gene expression was established through statistical analysis.【Results】The L value of the peach fruit color difference index increased gradually from 30 to 16 days before ripening and then decreased as the fruit matured.All the varieties exhibited an S-type a value trend,with a experiencing a sharp rise from 23 to 12 days prior to ripening.During the 12 days before ripening,the green hue of the peach fruit faded,and changed to red and showed a conti
作者
刘鑫
张晓煜
孟君仁
段文宜
孙世航
潘磊
曾文芳
王志强
牛良
LIU Xin;ZHANG Xiaoyu;MENG Junren;DUAN Wenyi;SUN Shihang;PAN Lei;ZENGWenfang;WANG Zhiqiang;NIU Liang(Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences/National Peach&Grape Improvement Center,Zhengzhou 450009,Henan,China;Zhongyuan Research Center,Chinese Academy of Agricultural Sciences,Xinxiang,453004,Henan,China)
出处
《果树学报》
CAS
CSCD
北大核心
2024年第6期1054-1063,共10页
Journal of Fruit Science
基金
国家桃产业技术体系(CARS-30)
中国农业科学院科技创新工程专项经费项目(CAAS-ASTIP-2023-ZFRI)
河南省重大公益科技专项(201300110500)。
