摘要
【目的】探究香榧Torreya grandis‘Merrillii’雌雄植株的生根差异和响应干旱胁迫的生理差异,为香榧抗干旱种质资源的培育提供科学依据。【方法】以香榧新生雌雄株枝条为材料,比较两者夏季扦插生根差异,对生根状况较一致的香榧雌雄株施加聚乙二醇(PEG)模拟干旱处理,比较不同时间下香榧雌雄株幼苗的抗氧化酶活性、渗透调节物质、膜脂过氧化和活性氧质量摩尔浓度的变化。【结果】夏季扦插的香榧雄株成活率普遍高于雌株,雌雄株扦插的最佳时间不同,与7和8月相比,6月扦插的香榧雌株成活率和生根率更高,侧根数更多,而8月扦插的香榧雄株较6和7月的成活率和生根率更高,侧根数更多。在扦插相同时间下,雄株的成活率和生根率均高于雌株。干旱胁迫引起香榧叶片抗氧化酶活性发生显著变化,并扰乱其渗透调节功能和活性氧代谢平衡。在干旱胁迫下,与雄株相比,雌株具有更高的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,以及较低的丙二醛(MDA)、脯氨酸(Pro)、超氧阴离子(O^(2−))和过氧化氢(H2O2)质量摩尔浓度。【结论】夏季扦插的香榧雄株比雌株具有更高的扦插成活率。干旱胁迫下,香榧雌株能够更好地利用抗氧化系统来减少干旱胁迫引起的活性氧积累和细胞损伤,从而使香榧雌株比雄株更耐干旱。
[Objective]This study,with an exploration of the rooting differences and the physiological differences in response to drought stress between male and female Torreya grandis‘Merrillii’,is aimed to provide a scientific basis for the cultivation of drought-resistant germplasm resources of T.grandis‘Merrillii’.[Method]First,with the shoots of newborn male and female T.grandis‘Merrillii’selected as materials,a comparison is conducted of the differences in rooting of summer cuttings between them.Then,PEG solution was applied to male and female T.grandis‘Merrillii’with consistent rooting status for simulated drought treatment after which the changes of antioxidant enzyme activity,osmomodulating substances,membrane lipid peroxidation and reactive oxygen species content of male and female seedlings were compared and analyzed under different treatment times.[Result]The survival rate of male T.grandis‘Merrillii’of summer cuttings was generally higher than female and the best time for cuttings was different for male and female T. grandis ‘Merrillii’. Cuttings for the female in June had higher survival, rooting rates and a higher number of lateral roots than those in July and August whereas the survival rate and rooting rate of male cuttings in August were higher than those in June and July, and the number of lateral roots was higher. When the male and female T. grandis ‘Merrillii’ were cut for the same number of days, the survival rate and rooting rate of male plants were higher than the female ones. Drought stress caused significant changes in antioxidant enzyme activity in fragrant leaves, and disturbed its osmoregulatory function and reactive oxygen species metabolism balance. Under drought stress, female plants had higher superoxide dismutase (SOD) activity, peroxidase (POD) activity and catalase (CAT) activity, as well as lower malondialdehyde (MDA), proline (Pro), superoxide anion (O2−) and hydrogen peroxide (H2O2) than male strains. [Conclusion] The male T. grandis ‘ Merrillii’ of sum
作者
汪小荣
邱红
张启香
吴家胜
娄和强
WANG Xiaorong;QIU Hong;ZHANG Qixiang;WU Jiasheng;LOU Heqiang(State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;College of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)
出处
《浙江农林大学学报》
CAS
CSCD
北大核心
2024年第3期478-485,共8页
Journal of Zhejiang A&F University
基金
浙江省农业新品种选育重大科技专项(2021C02066-11)。
关键词
香榧
雌雄异株植物
干旱胁迫
植物生理
Torreya grandis‘Merrillii’
dioecious plant
drought stress
plant physiology
