摘要
蛋白邻近标记(proximity labeling,PL)技术是指通过融合特定的催化酶,实现对目标蛋白相互作用蛋白的原位标记,再采用质谱或测序分析鉴定标记蛋白。该技术能够在瞬时动态条件下精确识别蛋白质相互作用,是传统分析手段的重要补充,在蛋白质相互作用研究中具有广阔应用前景。本文介绍了各类PL酶的工作原理、分类及优缺点;论述了该技术在研究蛋白质与蛋白质相互作用中的应用;讨论了PL技术的优势、目前存在的问题和发展趋势,以期为该技术的应用提供参考。
Proximity labeling(PL)technology refers to the in-situ labeling of protein-interacting proteins of interest by fusing specific catalytic enzymes,followed by mass spectrometry or sequencing analysis to identify the tagged proteins.This technique can accurately identify protein-protein interactions under transient dynamic condition,which is an important supplement to traditional analytical methods,and has a broad application prospect in the study of protein-protein interactions.In this paper,the working principle,classification,advantages and disadvantages of various PL enzymes were introduced,then the application of this technology in the study of protein-protein interactions was discussed,and finally the advantages,existing problems and development trends of adjacent labeling technology were discussed,in order to provide reference for the application of this technology.
作者
董娜
张文峰
DONG Na;ZHANG Wenfeng(School of Life Sciences and Biopharmaceutics,Guangdong Pharmaceutical University,Guangzhou 510006,China)
出处
《广东药科大学学报》
CAS
2024年第3期141-151,共11页
Journal of Guangdong Pharmaceutical University
基金
广州市科技计划项目(202201010036)
广东省基础与应用基础研究基金项目(2020A1515010889)。
