摘要
目的分析比较Cobas s201、Panther、ChiTaS BSS 1200/PreNAT II三套核酸筛查系统在温州地区无偿献血者中核酸检测(NAT)能力。方法本研究纳入了2018年1月~2023年5月在浙江温州地区无偿献血者586970份血液标本,应用了Cobas s201、Panther以及ChiTaS BSS 1200/PreNAT II三种核酸检测系统进行检测,Cobas s201系统采用6混样检测模式,Panther系统采用单检检测模式,ChiTaS BSS 1200/PreNAT II系统采用8混样/单检检测模式。结果Cobas s201、Panther、ChiTaS BSS 1200/PreNAT II三套系统阳性检出率分别为0.90‰(344/381919)、3.54‰(440/124303)和2.59‰(209/80748),且Panther系统的阳性检出率明显高于另外两套系统(P<0.05);温州地区NAT阳性主要为HBV感染,感染率为1.22‰(717/586970);42例NAT检测HBV阳性标本中主要为隐匿性感染。结论Cobas s201、Panther和ChiTaS BSS 1200/PreNAT II三套核酸筛查系统在温州地区献血者的酶免无反应性血液标本中均能有效检测出核酸阳性标本,从而降低ELISA检测出现的漏检风险,其中Panther系统表现出较高的阳性检出率,NAT系统在发现隐匿性HBV感染方面有显著作用,对于防止输血传播疾病具有重要意义。
Objective This study aims to evaluate and compare the efficacy of Cobas s201,Panther and ChiTas BSS 1200/PreNAT II's nucleic acid testing(NAT)systems in detecting blood donations in Wenzhou.Methods This study included 586970 blood samples collected from blood donors in Zhejiang Province,Wenzhou from January 2018 to May 2023,and detected by Cobas s201,Panther and ChiTaS BSS1200/PreNAT II nucleic acid detection systems.The three systems adopt different detection modes:the Cobas s201 system adopts a 6-sample detection mode;the Panther system adopts a single detection mode;and the ChiTaS BSS1200/PreNAT II system adopts an 8-sample/single detection mode.Results The positive detection rates of the three systems of Cobas s201,Panther and ChiTaS BSS1200/PreNAT II are:0.90%o(344/381919),3.54%o(440/124303)and 2.59%o(209/80748),and the positive detection rate of Panther is significantly higher,Compared with the other two systems(P<0.05);NAT-positive cases in Wenzhou area were mainly due to HBV infection,and the infection rate was 1.22%o(717/586970);among the 42 HBV-positive specimens tested by NAT,they were mainly occult infections.Conclusion The results of this study show that the three nucleic acid screening systems,Cobas s201,Panther and ChiTaS BSS1200/PreNAT I,can effectively detect nucleic acid-positive samples in enzyme immunoassay non-reactive blood samples from voluntary blood donors in Wenzhou,thereby reducing missed detections in ELISA testing.The Panther system showed a high positive detection rate,demonstrating its testing superiority in the region.In addition,NAT testing has a significant role in detecting occult HBV infection,which is of great significance in preventing transfusion-transmitted diseases.Therefore,regular analysis of test data,combined with the rational application of NAT detection systems,can further reduce the risk of transfusion-related infectious diseases.
作者
朱紫苗
蔡淑锋
刘燕飞
林碧
张锋
ZHU Zimiao;CAI Shufeng;LIU Yanfei;LIN Bi;ZHANG Feng(Central Blood Station of Wenzhou,Wenzhou 325000,China)
出处
《浙江实用医学》
2024年第2期102-105,121,共5页
Zhejiang Practical Medicine
关键词
献血者
温州地区
核酸筛查系统
酶联免疫吸附法
窗口期
输血安全
blood donors
Wenzhou region
nucleic acid screening system
enzyme linked immunosorbent assay
window period
transfusion safety
