摘要
目的调查鲁中地区禽源性大肠埃希菌mcr-1基因的携带情况,了解mcr-1基因阳性禽源性大肠埃希菌对常用β-内酰胺类药物、氨基糖苷类药物、喹诺酮类药物耐药基因的携带情况,掌握其耐药性。方法收集2020年4月至2021年10月鲁中地区3家大规模养殖场302份健康活鸡、鸭肛拭子新鲜粪便,对分离的大肠埃希菌用聚合酶链反应(PCR)检测mcr-1基因的携带率。对mcr-1阳性大肠埃希菌,采用BD凤凰hoenix TM 100 NMIC/ID-4复合板鉴定菌种及进行药敏试验,采用PCR检测各种β-内酰胺类药物耐药基因、氨基糖苷类修饰酶耐药基因、16S rRNA甲基化酶耐药基因和质粒介导的喹诺酮类药物耐药基因。结果302份样品中共分离出291株禽源性大肠埃希菌,其中27株携带mcr-1基因,mcr-1基因携带率为9.3%。27株mcr-1基因阳性禽源性大肠埃希菌中:超广谱β-内酰胺酶(ESBL)基因CTX-M-14、CTX-M-15、TEM-1携带率分别为100.00%(27/27)、70.37%(19/27)、74.07%(20/27);质粒介导AmpC酶耐药基因CMY-2携带率为14.81%(4/27);未检出bla SHV、bla DHA、OXA等β-内酰胺类药物相关耐药基因;未检出碳青霉烯酶基因;16S rRNA甲基化酶耐药基因rmtB及氨基糖苷类修饰酶耐药基因aac(6′)-Ⅰb-cr、ant(3″)-Ⅰ的携带率分别为25.93%(7/27)及25.93%(7/27)、92.59%(25/27);喹诺酮类药物耐药基因qnrS的携带率为81.48%(22/27),未检出qnrA、qnrB基因。对多黏菌素B、头孢噻肟、头孢西丁、左氧氟沙星、复方磺胺甲噁唑表现出了100.00%耐药,对头孢吡肟、头孢他啶、氨曲南、哌拉西林/他唑巴坦、阿米卡星和庆大霉素的耐药率分别为85.19%、33.33%、62.96%、14.81%、25.93%和77.78%,未出现对碳青霉烯类药物耐药的菌株。结论禽源性大肠埃希菌mcr-1基因的携带率为9.3%,是引起多黏菌素耐药的主要耐药机制。mcr-1基因阳性禽源性大肠埃希菌同时携带多种耐药基因,表现出多重耐药的特征。
Objective To investigate the carrying status of mcr-1 gene in avian Escherichia coli in the central area of Shandong,and to understand the drug resistance genes carrying status of mcr-1 gene positive avian Escherichia coli to commonly usedβ-lactam drugs,aminoglycosides and quinolones for mastering their drug resistance.Methods The anal swab fresh stool of 302 healthy live chickens and ducks from 3 large-scale farms in the central area of Shandong Province from April 2020 to October 2021 were collected.The carrying rate of mcr-1 gene in strains of isolated Escherichia coli was detected by PCR.For mcr-1 positive Escherichia coli,the BD Phoenix hoenix TM 100 NMIC/ID-4 composite plate was used to identify the bacterial species and conducted the drug susceptibility test.Variousβ-lactam drug resistance genes,minoglycoside modifying enzyme drug resistance genes,16S rRNA methylase drug resistance genes and plasmid-mediated quinolone drug resistance genes were detected by PCR.Results A total of 291 strains of avian Escherichia coli,were isolated from 302 samples,27 strains carried mcr-1 gene,with a carrying rate of 9.3%.Among 27 strains of mcr-1 positive avian Escherichia coli,the carrying rates of ultra-broad-spectrumβ-lactamase genes(ESBL)CTX-M-14,CTX-M-15 and TEM-1 were 100.00%(27/27),70.37%(19/27)and 74.07%(20/27)respectively;the carriage rate of CMY-2 in plasmid-mediated AmpC enzyme resistance genes was 14.81%(4/27);β-lactor-related resistance genes such as bla SHV,bla DHA and OXA were not detected;no carbapenem resistance genes were detected;the carrying rates of 16S rRNA methylase genes rmtB and aminoglycoside-modifying enzyme resistance genes aac(6′)-Ⅰb-cr,ant(3″)-Ⅰwere 25.93%(7/27),25.93%(7/27),92.59%(25/27)respectively;the carrying rate of quinolone resistance gene qnrS was 81.48%(22/27),qnrA and qnrB genes were not be detected.The drug resistance rates to polymyxin B,cefotaxime,cefoxitin,levofloxacin and cotrimoxazole were 100.00%.The drug resistance rates to cefepime,ceftazidime,amtraxam,piperacill
作者
刘丽娟
刘丽艳
张敏
翟伟
任玉国
LIU Lijuan;LIU Liyan;ZHANG Min;ZHAI Wei;REN Yuguo(Department of Clinical Laboratory,Affiliated People′s Hospital,Shandong First Medical University,Jinan,Shandong 250118,China;Department of Nephrology,Jinan Municipal Fifth People′s Hospital,Jinan,Shandong 250022,China)
出处
《检验医学与临床》
CAS
2024年第12期1676-1682,共7页
Laboratory Medicine and Clinic
基金
山东省医药卫生科技发展计划项目(2018WS293)
山东省济南市卫生健康委员会科技计划项目(2020-4-119)。
