摘要
目的:探讨补肺消结汤基于血管内皮生长因子(Vascular endothelial growth factor,VEGF)或VEGF受体-2(Vascular endothelial growth factor receptor-2,VEGFR-2)信号通路对肺癌大鼠的具体作用机制。方法:本研究通过建立A549肺癌大鼠移植瘤模型90例进行实验,随机分为对照组(n=30)、补肺消结汤低剂量组(n=30)及高剂量组(n=30),补肺消结汤低剂量组、高剂量组分别予以10mL/kg及20mL/kg剂量灌胃,对照组给予等量生理盐水灌胃,每组1次/天,连续持续14天。对肺组织进行病理组织学观察。同时,采用实时荧光定量PCR(Quantitative real-time PCR,qRT-PCR)检测肺癌组织中p53 mRNA表达情况,并用免疫印记方法(Western blot)检测大鼠肺组织中VEGF、VEGFR-2表达。结果:与对照组相比,低、高剂量组p53 mRNA水平表达显著升高(P<0.05)。与对照组相比,低、高剂量组VEGF、VEGFR-2表达显著降低(P<0.05)。结论:补肺消结汤可通过调控VEGF-VEGFR信号通路的基因表达,提高p53 mRNA表达水平,抑制大鼠的肺癌生长。
Objective:To investigate the Vascular endothelial growth factor(VEGF)or VEGF receptor-2(Vascular endothelial growth factor receptor-2)based on Bufei Xiaojie Decoction.The specific mechanism of VEGFR-2 signaling pathway on lung cancer rats.Methods:In this study,30 cases of transplanted tumor model of A549 lung cancer rats were established and randomly divided into control group(n=30),Bufei Xiaojie Decoction low-dose group(n=30)and high-dose group(n=30).Bufei Xiaojie Decoction low-dose group and high-dose group were given gavage of 10mL/kg and 20mL/kg,respectively.The control group was given the same amount of normal saline intragastrically,once a day in each group,for 14 days.The lung tissue was observed by histopathology.Meanwhile,Quantitative real-time PCR(qRT-PCR)was used to detect p53 mRNA expression in lung cancer tissue,and Western blot was used to detect VEGF and VEGFR-2 expression in rat lung tissue.Results:Compared with control group,p53 mRNA level in low and high dose groups was significantly increased(P<0.05).Compared with the control group,the expressions of VEGF and VEGFR-2 in low and high dose groups were significantly decreased(P<0.05).Conclusion:Bufei Xiaojie Decoction can regulate the gene expression of VEGF-VEGFR signaling pathway,increase the expression level of p53 mRNA,and inhibit the growth of lung cancer in rats.
作者
王斌灿
张鑫
陈家卫
陈志斌
Wang Bincan(Department of Respiratory and Critical Care Medicine,Fujian Second People’s Hospital,Fuzhou 350000)
出处
《黑龙江医药》
CAS
2024年第3期501-504,共4页
Heilongjiang Medicine journal
基金
福建省中青年教师教育科研项目(编号:JAT210179)。
