摘要
为了解主城区近年鸽散养户新城疫的免疫情况和养殖环境中的流行情况,2018—2023年分别采集4个主城区25个散养户的鸽血清样本1972份、鸽舍环境棉拭子150份、鸽双腔棉拭子750份,通过血凝及血凝抑制试验和荧光定量PCR的方法,进行新城疫抗体水平监测和新城疫病毒核酸检测。结果表明,乌鲁木齐市4个主城区免疫合格率呈逐年上升的趋势,仅2022年鸽新城疫免疫抗体均低于农业农村部规定的70%,其它均高于该标准;4个主城区中环境棉拭子新城疫病毒核酸群体阳性率2018—2023年分别为28%、20%、16%、28%、36%和20%;鸽双腔棉拭子新城疫病毒核酸个体阳性率2018—2023年分别为12.8%、11.2%、8.8%、8%、9.6%和4%。
In order to understand the immunity situation of pigeon free-range farmers and the epidemic situation of Newcastle disease in the breeding environment in the main urban area in recent years,from 2018 to 2023,1972 samples of pigeon serum,150 samples of pigeon shed environmental cotton swabs and 750 samples of pigeon double cavity cotton swabs were collected from 25 in 4 urban districts of Free-range farmers,the antibody level and nucleic acid of Newcastle disease virus(NDV)were detected by hemagglutination test(HA),hemagglutination inhibition test(HI)and fluorescence quantitative PCR(FQ-PCR).The results showed that the immunization qualification rate of 4 main urban areas in Urumqi was increasing year by year.In 2022,the immune antibody of pigeon Newcastle disease was lower than 70%of the standard set by the Ministry of Agriculture and rural areas,and the others were higher than the standard The positive rates of NDV DNA in environmental cotton swabs from 2018 to 2023 were 28%,20%,16%,28%,36%and 20%,respectively,in 4 urban districts,the positive rates of NDV DNA in pigeon swabs were 12.8%,11.2%,8.8%,8%,9.6%and 4%in 2018—2023.
作者
王涛
马佳妮
李兆新
姜玉婷
郭亚军
WANG Tao;MA Jia'ni;LI Zhaoxin;JIANG Yuting;GUO Yajun(Urumqi Animal Disease Control and Diagnosis Center,Urumqi Xinjiang 830063,China;Midong District Animal Husbandry and Veterinary Station,Urumqi Xinjiang 830017,China)
出处
《现代畜牧科技》
2024年第6期5-8,共4页
Modern Animal Husbandry Science & Technology
基金
新疆维吾尔自治区天山英才计划第三期培养人项目(新人社函[2021]37号)。
关键词
新城疫
抗体
效价
核酸检测
Newcastle disease
antibody
titer
nucleic acid testing
