摘要
目的:分析KIF11基因在肺腺癌组织的表达水平及其对肺腺癌患者生存预后的影响,初步探讨可能的作用机制。方法:利用生物信息学方法分析TCGA和GEO数据库中肺腺癌患者KIF11 mRNA表达及信号通路富集情况。采用Kaplan-Meier曲线分析KIF11 mRNA的表达水平对患者总生存时间的影响。利用单因素及多因素Cox回归分析方法分析TCGA数据库中影响肺腺癌患者预后的因素,并评估KIF11与Ki-67表达水平的关系。收集60对某院收治的肺腺癌患者癌组织及癌旁正常组织标本,利用免疫组化方法检测KIF11蛋白和Ki-67表达水平,分析KIF11蛋白表达水平与患者临床病理因素的关联性。流式细胞术检测KIF11对肺腺癌细胞周期分布的影响。结果:TCGA和GEO数据库中癌组织KIF11 mRNA表达水平均高于正常组织,差异有统计学意义(P<0.05)。Kaplan-Meier生存分析结果显示,KIF11 mRNA高表达组患者生存时间短于低表达组(P<0.05)。免疫组化结果显示,肺腺癌患者癌组织KIF11和Ki-67蛋白表达均高于癌旁组织,差异有统计学意义(P<0.05)。多因素Cox回归分析显示,肺腺癌的预后与T分期、是否淋巴结转移及KIF11 mRNA表达水平有关。KIF11 mRNA高表达组肿瘤直径>3 cm、发生淋巴结转移、分化程度为高分化、Ki-67高表达患者比例高于KIF11 mRNA低表达组,肿瘤分期为Ⅰ期的患者比例低于KIF11 mRNA低表达组,差异均有统计学意义(P<0.05)。KIF11蛋白与Ki-67表达水平呈正相关(P<0.05)。KIF11基因GO富集及KEGG分析结果显示,KIF11基因在细胞分裂、DNA复制信号通路上功能富集。基因干扰试验显示,干扰肺腺癌细胞A549及H1975细胞的KIF11基因表达后,肺腺癌细胞的G1期细胞比例增加,S期细胞比例减少(P<0.05)。结论:肺腺癌组织KIF11表达水平明显高于正常组织,其高表达与肺腺癌患者预后有关,可能通过调节肺腺癌细胞周期、促进细胞增殖机制发挥作用,KIF11可作为肺腺癌�
Objective:To study the expression level and prognostic significance of KIF11 gene in lung adenocarcinoma tissue and explore its possible mechanism of KIF11 in the cancer.Methods:Bioinformatics methods were used to analyze KIF11 mRNA expression and signaling pathway enrichment in lung adenocarcinoma patients in TCGA and GEO databases.Kaplan-Meier curve was applied to analyze the impact of KIF11 mRNA expression level on patients’overall survival time.Single-factor and multi-factor Cox regression analysis methods were taken to analyze the factors affecting the prognosis of patients with lung adenocarcinoma in the TCGA database,and the relationship between the expression levels of KIF11 and Ki-67 in the TCGA database was assessed.Lung cancer tissues and adjacent normal tissue specimens from 60 pairs of lung adenocarcinoma patients admitted to a hospital were collected.Immunohistochemistry was used to detect the expressions of KIF11 protein and Ki-67,and the correlation between KIF11 protein expression level and various clinicopathological factors of the patients was analyzed.Flow cytometry was adopted to detect the effect of KIF11 on the cycle distribution of lung adenocarcinoma cells.Results:The expression levels of KIF11 mRNA in cancer tissues in the TCGA database and GEO database were higher than those in the normal tissues,the differences being statistically significant(P<0.05).Kaplan-Meier survival analysis results showed that the survival time of the patients in the high expression group of KIF11 mRNA was shorter than that of the patients in the lower expression group(P<0.05).Immunohistochemistry result denoted that the protein expressions of KIF11 and Ki-67 in lung cancer tissues were all higher than those in adjacent tissues(P<0.05).Univariate and multivariate Cox regression analysis indicated that there was a significant statistical difference between the expression level of KIF11 mRNA and tumor size,lymph node metastasis,T stage,degree of differentiation and Ki-67 expression level,indicating a poor progno
作者
陶累累
陈平
马靓
严文跃
钱琦
赵霞
TAO Leilei;CHEN Ping;MA Liang;YAN Wenyue;QIAN Qi;ZHAO Xia(Department of Oncology,The First People’s Hospital of Yancheng City,Jiangsu 224008,China)
出处
《淮海医药》
CAS
2024年第3期221-226,共6页
Journal of Huaihai Medicine
基金
2020年盐城市医学科技发展计划项目(YK2020024)。
关键词
肺腺癌
KIF11
表达水平
预后
细胞周期
Lung adenocarcinoma
KIF11
Expression level
Prognosis
Cell cycle
