摘要
背景:子宫螺旋动脉重铸的顺利进行是正常妊娠的必要条件之一,血管平滑肌细胞是此过程的重要细胞。干扰素γ与妊娠早期螺旋动脉血管平滑肌细胞丢失相关,但具体机制尚未明确。目的:探讨干扰素γ通过NLRP3/caspase-1/GSDMD焦亡途径对血管平滑肌细胞迁移、凋亡生物学功能的影响。方法:人血管平滑肌细胞分为对照组和干扰素γ组,对照组正常培养,干扰素γ组采用10 ng/mL干扰素γ处理24 h。Transwell实验检测血管平滑肌细胞的迁移能力;荧光TUNEL实验及流式细胞术检测血管平滑肌细胞的凋亡情况;qPCR检测NLRP3和caspase-1 mRNA表达水平;Western blot检测NLRP3、caspase-1、cleaved N-terminal GSDMD蛋白表达水平。结果与结论:与对照组相比,干扰素γ组血管平滑肌细胞的迁移能力及凋亡率显著升高(P<0.05);与对照组相比,干扰素γ组血管平滑肌细胞中NLRP3和caspase-1 mRNA表达水平显著升高(P<0.05);与对照组相比,干扰素γ组血管平滑肌细胞中NLRP3、caspase-1、cleaved N-terminal GSDMD蛋白表达水平显著升高(P<0.05)。结果表明,干扰素γ可能通过NLRP3/caspase-1/GSDMD焦亡途径调控血管平滑肌细胞的迁移和凋亡。
BACKGROUND:Successful uterine spiral artery remodeling is necessary for normal pregnancy,in which vascular smooth muscle cells are important cells.Interferon-γis associated with the loss of vascular smooth muscle cells during early pregnancy.However,the specific mechanism is not fully understood.OBJECTIVE:To investigate the effects of interferon-γon migration and apoptosis of vascular smooth muscle cells through NLRP3/caspase-1/GSDMD pyroptosis pathway.METHODS:Human vascular smooth muscle cells were divided into control group and interferon-γgroup.The control group was cultured normally,and the interferon-γgroup was treated with 10 ng/mL interferon-γfor 24 hours.The migration ability of vascular smooth muscle cells was detected by Transwell assay.The apoptosis of vascular smooth muscle cells was detected by TUNEL assay and flow cytometry.The mRNA expression levels of NLRP3 and caspase-1 were detected by qPCR.Western blot assay was utilized to detect NLRP3,caspase-1,and cleaved N-terminal GSDMD protein expression levels.RESULTS AND CONCLUSION:Compared with the control group,the migration ability and apoptosis rate of vascular smooth muscle cells in interferon-γgroup were significantly increased(P<0.05).Compared with the control group,the mRNA expression levels of NLRP3 and caspase-1 in vascular smooth muscle cells of interferon-γgroup were significantly increased(P<0.05).Compared with control group,the expression levels of NLRP3,caspase-1,and cleaved N-terminal GSDMD protein in vascular smooth muscle cells in the interferon-γgroup were significantly increased(P<0.05).The results suggest that interferon-γmay regulate the migration and apoptosis of vascular smooth muscle cells through NLRP3/caspase-1/GSDMD pyroptosis pathway.
作者
万玲玲
吴梦滢
张宇骄
罗青清
Wan Lingling;Wu Mengying;Zhang Yujiao;Luo Qingqing(Department of Obstetrics,The Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China;Department of Obstetrics and Gynecology,First Affiliated Hospital of Suzhou University,Suzhou 215006,Jiangsu Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2025年第7期1422-1428,共7页
Chinese Journal of Tissue Engineering Research
基金
泸州市社会发展重点科技专项(2021-SYF-27),项目负责人:罗青清
泸州市社会发展重点研究项目(2021-JYJ-56),项目负责人:张宇骄
四川省自然科学基金项目(2022NSFSC1373),项目负责人:罗青清
西南医科大学附属医院博士科研启动基金(21055),项目负责人:罗青清。
