摘要
目的建立micro-CT和小动物高频超声成像系统评价实验性类风湿关节炎研究标准。方法分别构建5只IL-1β拮抗剂敲除自发性关节炎小鼠和使用牛Ⅱ型胶原诱导为类风湿性关节炎(CIA)的SD大鼠。应用micro-CT对关节炎模型鼠的足趾进行成像扫描,分析骨体积分数(BV/TV)和骨表面积和组织体积的比值(BS/TV)等微观参数。使用小动物高频超声成像系统对关节炎模型鼠的膝关节进行成像,检测膝关节的回声强度,并观察膝关节是否有积液。结果Micro-CT可以清楚地检测到关节炎模型鼠的足趾部位膨大和畸形,微观参数BV/TV和BS/TV显著降低(P<0.05)。小动物高频超声成像系统可以清楚地观察到关节炎模型鼠膝关节存在积液,关节炎模型组膝关节灰度值量化反映的回声强度显著低于对照组(P<0.05)。结论应用micro-CT和小动物高频超声成像系统分别对小动物的足趾和膝关节成像分析,可以系统地评价实验性类风湿关节炎的发展水平,弥补实验性RA成像缺乏统一标准的缺陷。
Objective To establish the evaluation criteria of experimental rheumatoid arthritis by micro-CT and high-frequency ultrasound.Methods Five IL-1βknockout mice with spontaneous arthritis and five SD rats with rheumatoid arthritis(CIA)induced by bovine typeⅡcollagen were constructed.Then,the toes of arthritis model mice were imaged by micro-CT,and the microscopic parameters such as BV/TV and BS/TV were analyzed.High-frequency ultrasound was used to image the knee joint of arthritis model mice to detect the echo intensity of the knee joint and observe whether there was effusion in the knee joint.Results Micro-CT could clearly detect the enlargement and deformity of the toes in the arthritis model mice,and the microscopic parameters BV/TV and BS/TV were significantly reduced.High-frequency ultrasound could clearly observe the knee joint effusion in arthritis model mice,and the echo intensity of knee joint gray value quantitative response in arthritis model group was significantly lower than that in control group.Conclusion The application of micro-CT and high-frequency ultrasound imaging analysis of small animal toes and knees can systematically evaluate the development of experimental rheumatoid arthritis,and compensate for the lack of unified standards in experimental rheumatoid arthritis imaging.
作者
次旦南卓
侯豹
黄术兵
蔡维维
孙海建
米玛
邱丽颖
TSEDIEN Nhamdriel;HOU Bao;HUANG Shubing;CAI Weiwei;SUN Haijian;Mi Ma;QIU Liying(University of Tibetan Medicine,Lhasa 850000,China;Wuxi Medical College,Jiangnan University,Wuxi 214122,China)
出处
《分子影像学杂志》
2024年第5期502-506,共5页
Journal of Molecular Imaging
基金
藏医药“十四五”规划内涵建设项目(2023ZYYGH04)。
