摘要
为了研究核电站温排水污染对水生动物长期胁迫的影响,实验通过室内模拟核电站温排水的游离余氯残留和温升效应,研究了不同温度和不同游离余氯浓度耦合下对泥蚶的氧化胁迫效应。设置水温为19和29℃,游离余氯浓度分别为0.33和6.66 mg/L,进行为期20 d的胁迫实验。结果显示:①单一温升条件下,温度升高可以显著影响泥蚶ROS水平。温度越高,ROS水平越高。②0.33 mg/L浓度组泥蚶ROS水平和DNA损伤在第20天时显著升高,19和29℃下分别为对照组的2.2、2.9倍和1.8、2.2倍;细胞活性、SOD活性、ATP酶活性均下降;6.66 mg/L浓度组ROS水平在第20天时显著上升,19和29℃下分别为对照组的6.2、3.6倍,DNA损伤增加,细胞活性、血细胞吞噬率和ATP酶活性下降,SOD酶活性在第20天、29℃时降至最小值(36.4±8.4)U/(mg·prot)。③双因素方差分析结果显示,水温和游离余氯对泥蚶ROS水平、DNA损伤、血细胞吞噬活性、ATP酶活性具有显著交互作用。研究表明,游离余氯能够显著增加泥蚶ROS水平和DNA损伤,温度升高和游离余氯复合暴露对泥蚶造成更大的胁迫压力,影响了其机体内氧化响应相关生理过程,余氯排放海域夏季相比冬季会对泥蚶产生更大的氧化胁迫。本研究可为温度升高和游离余氯复合污染中的海洋生物氧化胁迫毒性研究提供参考,并且为生态风险评估提供科学依据。
To investigate the effects of warm waste water from nuclear power plants on the long-term stress of aquatic animals,the influence of free residual chlorine on the oxidative pressure of Tegillarca granosa under different temperature conditions were studied through laboratory simulation.Water temperatures were set as 19 and 29℃,and two different gradients of free residual chlorine concentration were set as 0.33 mg/L and 6.66 mg/L.The results showed that:①water temperature can significantly affect the ROS level.The higher the temperature,the higher the ROS level.②the ROS level and DNA damage of T.granosa in 0.33 mg/L group were increased significantly on day 20,which were 2.2,2.9 times and 1.8,2.2 times higher than those in the control group at 19 and 29℃,respectively;the cell activity,ATPase activity and SOD enzyme activity decreased significantly;the ROS level in the 6.66 mg/L group increased significantly on day 20,which was 6.2 and 3.6 times higher than that in the control group at 19℃ and 29℃,respectively;the DNA damage increased;the cell activity,hemocyte phagocytosis rate and ATPase activity decreased;SOD enzyme activity decreased to the minimum value of(36.4±8.4)U/(mg prot)at 29℃ on day 20.③two-factor analysis of variance showed that water temperature and free residual chlorine had significant interaction on the parameters of ROS level,DNA damage,hemocyte phagocytosis rate and ATPase activity.The results indicate that free residual chlorine can significantly increase the ROS level and the DNA damage of T.granosa.The combined exposure of water temperature and residual chlorine,which caused greater stress pressure on T.granosa,affected the physiological processes related to oxidative stress.In the residual chlorine discharge area,greater oxidative stress would be faced by T.granosa in summer than in winter.This study can give a reference for studying oxidative stress toxicity of marine organisms exposed to warm water combined with residual chlorine,and provide scientific basis for ecological r
作者
陈琳
滕爽爽
胡高宇
肖国强
黄晓林
CHEN Lin;TENG Shuangshuang;HU Gaoyu;XIAO Guoqiang;HUANG Xiaolin(Wenzhou Key Laboratory of Marine Biological Genetics and Breeding,Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-Resource,Zhejiang Mariculture Research Institute,Wenzhou 325005,China;National Engineering Research Center for Marine Aquaculture,Zhejiang Ocean University,Zhoushan 316022,China)
出处
《水产学报》
CAS
CSCD
北大核心
2024年第6期24-36,共13页
Journal of Fisheries of China
基金
浙江省重点研发计划项目(2019C02045)
现代农业产业技术体系建设专项(CARS-49)
台州市重大农业科技“揭榜挂帅”研发攻关计划(NYJBGS202203)。
关键词
泥蚶
核电站温排水
温度升高
游离余氯
氧化响应
活性氧
DNA损伤
Tegillarca granosa
warm drainage pollution of nuclear power plant
temperature rise
free residual chlorine
oxidative response
ROS
DNA damage
