摘要
目的:利用报告菌株紫色杆菌探究草莓酸的群体感应抑制性及其对莓实假单胞菌的群体感应作用。方法:利用细菌凋亡率、气相色谱-质谱分析、生物膜的抑制效果、胞外聚合物生成量以及观察细菌的群集和泳动等参数,来研究草莓酸对莓实假单胞菌群体感应的抑制效果。结果:草莓酸的最小抑菌浓度(MIC)为0.5 mg/mL,亚抑菌浓度下草莓酸虽然降低了菌株CV026产生紫色素的能力,但对细胞凋亡没有任何影响。通过气相色谱-质谱定量检测发现莓实假单胞菌所产的群体感应信号分子是C8-HSL和C10-HSL,草莓酸对信号分子的分泌产生抑制效果。在亚抑菌浓度下草莓酸对莓实假单胞菌的生物被膜形成量为21.06%,细菌的群集和泳动的抑制率分别降低34.06%和75.02%,且呈现质量浓度依赖性。结论:草莓酸具有较好的群体感应抑制性,可以作为水产保鲜中的群体感应抑制剂。
In order to investigate the quorum-sensing inhibition of 2-methyl-2-pentenoic acid and its quorum-sensing effect on Pseudomonas fragi by using the reporter strain Chromobacterium violaceum 026(CV026).The inhibition effect of 2-methyl-2-pentenoic acid on the population induction of P.fragi was analyzed by bacterial apoptosis rate,gas chromatography-mass spectrometry analysis,biofilm inhibition effect,the amount of extracellular polymer formed,bacterial swarming and swimming.It was demonstrated that the minimum inhibitory concentration of 2-methyl-2-pentenoic acid was 0.5 mg/mL,and 2-methyl-2-pentenoic acid could reduce the ability of CV026 to produce purple bacteriocin at sub-inhibitory concentration and did not affect the apoptosis.The population-sensing signal molecules C8-HSL and C10-HSL produced by P.fragi were detected quantitatively by gas chromatography-mass spectrometry,and 2-methyl-2-pentenoic acid had an inhibitory effect on the secretion of the produced signal molecules.The biofilm formation of P.fragi by subinhibitory concentration 2-methyl-2-pentenoic acid was 21.06%,and the inhibition of bacterial swarming and swimming was reduced by 34.06%and 75.02%,respectively,in a concentration-dependent manner.In conclusion,2-methyl-2-pentenoic acid had good population induction inhibitory property and could be used as a population induction inhibitor in aquatic preservation.
作者
崔方超
王芊芊
刘佳宜
王当丰
檀茜倩
李秋莹
励建荣
李婷婷
Cui Fangchao;Wang Qianqian;Liu Jiayi;Wang Dangfeng;Tan Xiqian;Li Qiuying;Li Jianrong;Li Tingting(College of Food Science and Engineering,Bohai University,National&Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,China Light Industry Key Laboratory of Marine Fish Processing,Jinzhou 121013,Liaoning;College of Life Science,Dalian Minzu University,Dalian 116600,Liaoning)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2024年第5期171-181,共11页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金重点项目(U20A2067)
辽宁省自然科学基金博士科研启动基金计划项目(2022-BS-301)。
关键词
草莓酸
莓实假单胞菌
群体感应
抑制剂
2-methyl-2-pentenoic acid
Pseudomonas fragi
quorum sensing
inhibitors
