摘要
该文以3,3',5,5'-四甲基联苯胺(TMB)和罗丹明B为比色探针,建立了α-葡萄糖苷酶的比色检测方法,并在此基础上基于智能手机策略,通过RGB值对α-葡萄糖苷酶进行检测。结果表明,Fe^(3+)-TMB-罗丹明B显色体系的吸光度与α-葡萄糖苷浓度在20~100 U/L范围内呈线性关系,检出限为3.3 U/L。采用photo⁃shop软件分析样品图像和样品荧光图像的RGB值,其与α-葡萄糖苷酶浓度在10~100 U/L范围内呈线性关系,检出限分别为2.5 U/L和1.2 U/L。该法已成功用于α-葡萄糖苷酶抑制剂阿卡波糖抑制率评估和胎牛血清中α-葡萄糖苷酶浓度的测定。所构建的智能手机辅助检测法为α-葡萄糖苷酶的临床分析和酶抑制剂的快速筛选提供了简便、快捷和准确的方法。
This study exploited 3,3',5,5'-tetramethylbenzidine(TMB)and Rhodamine B as chromogenic probes forα-glucosidase concentration analysis via a colorimetric detection method.On this basis,a smartphone assisted strategy was designed to detectα-glucosidase activity by RGB value analysis of images.The results showed that the absorbance of Fe^(3+)-TMB-Rhodamine B system was linearly related toα-glucosidase concentration in the range of 20-100 U/L,and the detection limit was 3.3 U/L.The RGB values of the sample photos and fluorescence photos were analyzed by photo⁃shop,which showed a linear relationship withα-glucosidase activity in the range of 10-100 U/L,the detection limits were 2.5 U/L and 1.2 U/L forα-glucosidase,respectively.The method has been successfully used to detectα-glucosidase inhibitor acarbose and to quantitatively detectα-glucosidase activity in spiked fetal bovine serum.The smartphone assisted detection method provided a simple,quick and accurate method for clinical analysis ofα-glucosidase and rapid screening of enzyme inhibi⁃tors.
作者
赵灵芝
赵柳
张小清
寇张文
魏世楠
ZHAO Ling-zhi;ZHAO Liu;ZHANG Xiao-qing;KOU Zhang-wen;WEI Shi-nan(Department of Pharmacy,Xi'an Medical University,Xi'an 710021,China;Shaanxi Agricultural Inspection and Testing Center,Xi'an 710003,China;Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province,School of Chemistry and Chemical Engineering,Shaanxi Normal University,Xi'an 710062,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2024年第6期905-912,共8页
Journal of Instrumental Analysis
基金
陕西省科技厅项目(2022JM-094,2021JM-502)
西安医学院杰出人才青年计划配套项目(2021PT08)
2022年科研能力提升计划项目(2022NLTS072)
陕西省科技厅社发项目(2024SF-YBXM-661)
咸阳市科技局社发项目(L2023-ZDYF-SF-023)。
