摘要
目的:探究培养液中添加玉竹多糖对人脐带间质干细胞(hUC-MSCs)培养的影响。方法:比较在培养液中添加玉竹多糖和传统培养方法得到的人脐带间质干细胞,在形态、稳定性、产量、细胞分化等指标上的区别。结果:测得传统酶消化法P0代细胞每瓶(T75)收获细胞总量为4.53×10^(6)cells、活率95.5%,而添加玉竹多糖的酶消化法收获细胞总量为5.35×10^(7)cells、活率97.9%;定向诱导分化两种方法得到的hUC-MSCs,无明显差异。结论:与传统酶消化法相比,添加玉竹多糖的酶消化法可以提高细胞产量、缩短生产时间,利于细胞的大规模化生产,且细胞分化无明显差异,能满足生产和临床需求。
Objective:To investigate the effect of polysaccharide of Polygonatum odoratum in culture medium on human umbilical cord mesenchymal stem cells(hUC-MSCs)culture.Methods:Compare the morphological,stability and yield of human umbilical cord mesenchymal stem cells obtained by adding polysaccharide of Polygonatum odoratum in culture medium and traditional culture method.Results:The total number of cells harvested per vial(T75)by the traditional enzyme digestion method is 4.53×10^(6)cells and the viability rate is 95.5%,while the total number of cells harvested by the enzyme digestion method with polysaccharide of Polygonatum odoratum is 5.35×10^(7)cells and the viability rate is 97.9%;there is no significant difference between hUC-MSCs obtained by the two methods of directional induction differentiation.Conclusion:Compared with the traditional enzyme digestion method,the enzyme digestion method with polysaccharide of Polygonatum odoratum can increase the cell yield and shorten the production time,which is conducive to the large-scale production of cells,and there is no significant difference in cell differentiation,which could meet the production and clinical needs.
作者
宋泓纬
高业胜
江晔
SONG Hongwei;GAO Yesheng;JIANG Ye(College of Biological Science and Technology,Hunan Agricultural University,Changsha 410000,China;Huayan Pharmaceutical Technology Co.,Ltd.,Shanghai 200020,China)
出处
《生物化工》
CAS
2024年第2期63-67,共5页
Biological Chemical Engineering
关键词
人脐带间充质干细胞
细胞培养
酶消化法
玉竹多糖
human umbilical cord mesenchymal stem cells
cell culture
enzyme digestion method
polysaccharide of Polygonatum odoratum
