摘要
为探明烟草不同抗性品种NC95和SY04-3对马铃薯Y病毒(PVY)胁迫的响应差异,对烟草幼苗接种PVY,在12 h采集幼苗样本,进行转录组测序和生物信息学分析。结果表明:NC95上调差异表达基因有206个,下调111个;SY04-3上调差异表达基因有1001个,下调423个。这些差异表达基因的功能归类于生物过程、细胞组分及分子功能3大类54个GO条目,NC95和SY04-3上调差异表达基因显著富集在7条KEGG代谢通路中,而NC95和SY04-3下调差异表达基因分别显著富集在10条、14条KEGG代谢通路中;转录因子预测NC95有13个家族的20个转录因子发生显著变化,SY04-3有20个家族的38个转录因子发生了显著变化。
In order to explore the response differences of different tobacco resistant varieties NC95and SY04-3 to potato virus Y stress,tobacco seedlings were inoculated with PVY,and seedling samples were collected at 12 h.Transcriptome sequencing and bioinformatics analysis were performed.The analysis results of PVY inoculation and control showed that NC95 up-regulated 206 differentially expressed genes and down-regulated 111 genes.SY04-3 up-regulated 1001 differentially expressed genes,down-regulated 423.The functions of these differentially expressed genes are classified into 54 GO entries in three categories:biological processes,cell components and molecular functions.NC95 and SY04-3 up-regulated differentially expressed genes were significantly enriched in 7KEGG metabolic pathways,while NC95 and SY04-3 down-regulated differentially expressed genes were significantly enriched in 10 and 14 KEGG metabolic pathways,respectively;Transcription factors predicted that 20 transcription factors in 13 families of NC95 changed significantly,and 38 transcription factors in 20 families of SY04-3 changed significantly.This study provides reference for the study on the early response of tobacco to potato Y virus infection mechanism.
作者
王平
任雅萍
任昭辉
苏亮
吴委林
杨超群
董环宇
朴世领
WANG Ping;REN Yaping;REN Zhaohui;SU Liang;WU Weilin;YANG Chaoqun;DONG Huanyu;PIAO Shiling(College of Agonomy,Yanbian University,Yanji 133002,China;Hulunbuir Institute of Agriculture and Animal Husbandry,Hulunbuir 021000,China;Jilin Tobacco Industrial Co.,Ltd.,Changchun 130031,China)
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2024年第2期219-227,共9页
Journal of Jilin Agricultural University
基金
国家自然科学基金项目(31760418)。
关键词
烟草
马铃薯Y病毒
转录组
差异表达基因
tobacco
potato virus Y
transcriptome
dfferentially expressed genes
