摘要
目的基于气相色谱-质谱联用技术(GC-MS)的代谢组学方法研究博落回急性染毒模型大鼠心肌代谢产物的变化,寻找体内特征性代谢产物并探索相关毒性机制。方法博落回总碱提取物溶液灌胃给药,剂量382 mg/kg,对照组给予相同剂量的空白溶液,利用GC-MS方法对心肌样本进行分析,利用偏最小二乘法-判别分析对结果进行模式识别,通过VIP(The variable importance in the projection)值>1以及Student’s t检验(P<0.01)筛选出具有特征改变的差异代谢产物。结果与对照组相比较,发现21种潜在的特征性代谢产物,经KEGG通路富集分析发现,这些代谢产物主要参与甘氨酸、丝氨酸和苏氨酸代谢途径、甘油酯代谢途径以及丙酮酸代谢途径。结论经过对博落回染毒大鼠心肌代谢研究,发现了与中毒密切相关的代谢产物信息,为其中毒机制研究及法医学鉴定提供参考。
Objective To analyze the metabolic changes of myocardial tissue in rats under acute exposure to macleaya cordata by gas chromatography mass spectrometry(GC-MS),explore forensic identifications of its characteristic metabolites,and verify its toxicological mechanism in poisoning cases.Methods The rats in the exposure group were given 382 mg/kg macleaya extract solution by gavage,and the rats in the control group were given the same dose of solvent.The myocardial samples were analyzed by GC-MS,and pattern recognition was conducted through partial least squares discriminant analysis(PLSDA).The differential metabolites with characteristic changes were identified by variable importance projection(VIP value>1)and Student's t test(P<0.01).Results Compared with the control group,21 potential characteristic metabolites were identifed.Through KEGG pathway enrichment analysis,it was found that these metabolites were mainly involved in the pathways of glycine,serine and threonine metabolism;pyruvate metabolism and glycerolipid metabolism.Conclusion Through the study of myocardial metabolism in rats exposed to macleaya cordata,we found the information on metabolites closely related to poisoning,which provides new insight and reference for studies on the mechanisms of macleaya cordata poisoning in the field of forensic medicine.
作者
方世勇
石晓玲
张静
卢玉坤
方培
陈光
吴茂旺
徐祥
Fang Shiyong;Shi Xiaoling;Zhang Jing;Lu Yukun;Fang Pei;Chen Guang;Wu Maowang;Xu Xiang(School of Forensic Medicine,Wannan Medical College,Wuhu 241000,China;Anhui Health College,Chizhou 247000,China)
出处
《中国法医学杂志》
CSCD
2024年第1期39-43,共5页
Chinese Journal of Forensic Medicine
基金
公安部法医病理学重点实验室开放课题(GABFYBL201803)
安徽省高校科学研究项目(2022AH051222)
皖南医学院校中青年科研基金(WK202105)
安徽省大学生创新创业训练计划项目(S202210368007)。
关键词
法医毒理学
毒性
代谢组学
博落回
气相色谱-质谱联用法
Forensic toxicology
Toxicity
Metabolomics
Macleaya cordata
Gas chromatography-mass spectrometry
