摘要
目的分析DNA甲基转移酶1(DNMT1)与T淋巴细胞因子在急性髓系白血病(AML)中的表达水平及相关性。方法选取昆明医科大学第一附属医院2020年1月至2022年12月收治的初诊AML患者作为AML组(38例),另选取同期在昆明医科大学第一附属医院进行骨髓穿刺的缺铁性贫血患者作为对照组(21例)。检测并比较两组骨髓DNMT1的信使RNA(mRNA)表达水平及T淋巴细胞因子[白细胞介素(IL)-2、IL-4、IL-6、IL-10、干扰素-γ(INF-γ)、肿瘤坏死因子-α(TNF-α)、转化生长因子-β(TGF-β)]水平,以及比较不同临床特征AML患者DNMT1表达水平。采用Pearson相关分析AML患者DNMT1表达水平与T淋巴细胞因子水平的相关性。绘制受试者工作特征(ROC)曲线分析DNMT1、T淋巴细胞因子单独及联合检测对AML的诊断效能。结果AML组DNMT1 mRNA表达水平高于对照组(P<0.001)。白细胞计数(WBC)≥5.0×10^(10)/L、骨髓幼稚细胞比例≥60%、外周血幼稚细胞比例≥60%、乳酸脱氢酶(LDH)≥300 U/L、骨髓外浸润、染色体核型预后不良患者DNMT1表达水平分别高于WBC<5.0×10^(10)/L、骨髓幼稚细胞比例<60%、外周血幼稚细胞比例<60%、LDH<300 U/L、无骨髓外浸润、染色体核型预后良好患者,差异均有统计学意义(P<0.05)。AML组血清IL-2、IL-4、IL-10、INF-γ水平高于对照组,TGF-β水平低于对照组,差异均有统计学意义(P<0.05)。Pearson相关分析结果显示,DNMT1表达水平与IL-2、IL-4、IL-10、INF-γ水平呈正相关(r=0.574、0.619、0.527、0.483,P<0.05),与TGF-β水平呈负相关(r=-0.475,P<0.05)。ROC曲线分析结果显示,DNMT1联合IL-2、IL-4、IL-10、INF-γ检测诊断AML的曲线下面积分别为0.918、0.711、0.756、0.726。结论AML患者的DNMT1表达水平升高、T淋巴细胞因子表达失衡,二者存在一定相关性,DNMT1与部分T淋巴细胞因子联合检测对AML具有诊断价值。
Objective To analyze the expression levels of DNA methyltransferase 1(DNMT1)and T lymphocyte cytokines in acute myeloid leukemia(AML)and their correlation.Methods A total of 38 patients with newly diagnosed AML admitted to the First Affiliated Hospital of Kunming Medical University from January 2020 to December 2022 were selected as the AML group,and 21 patients with iron deficiney anemia who underwent bone marrow aspiration in the First Affiliated Hospital of Kunming Medical University during the same period were selected as the control group.The expression level of DNMT1 messenger RNA(mRNA)in bone marrow and T lymphocyte cytokines[interleukin(IL)-2,IL-4,IL-6,IL-10,interferon-γ(INF-γ),tumor necrosis factor-α(TNF-α),transforming growth factor-β(TGF-β)]levels were detected and compared between the two groups.The expression levels of DNMT1 in AML patients with different clinical characteristics were compared.Pearson correlation analysis was used to analyze the correlation between DNMT1 expression level and T lymphocyte cytokines levels in AML patients.The receiver operating characteristic(ROC)curve was drawn to analyze the diagnostic efficacy of DNMT1,T lymphocyte cytokines alone and combined detection in AML.Results The expression level of DNMT1 mRNA in AML group was significantly higher than that in control group(P<0.001).The expression levels of DNMT1 in patients with white blood cell count(WBC)≥5.0×10^(10)/L,bone marrow blast cell ratio≥60%,peripheral blood blast cell ratio≥60%,lactate dehydrogenase(LDH)≥300 U/L,bone marrow infiltration and chromosome karyotype poor prognosis were higher than those with WBC<5.0×10^(10)/L,bone marrow blast cell ratio<60%,peripheral blood blast cell ratio<60%,LDH<300 U/L,no bone marrow infiltration and chromosome karyotype good prognosis,and the differences were statistically significant(P<0.05).The levels of serum IL-2,IL-4,IL-10 and INF-γin AML group were higher than those in control group,and the level of TGF-βin AML group was lower than that in control group
作者
许晨晨
李轶勋
李姿璇
程沈菊
史明霞
武坤
XU Chenchen;LI Yixun;LI Zixuan;CHENG Shenju;SHI Mingxia;WU Kun(Department of Hematology,the First Affiliated Hospital of Kunming Medical University/Yunnan Blood Disease Research Center,Kunming,Yunnan 650032,China;Department of Medical Laboratory,the First Affiliated Hospital of Kunming Medical University/Yunnan Provincial Key Laboratory of Laboratory Medicine/Yunnan Provincial Medical Laboratory Clinical Medicine Research Center,Kunming,Yunnan 650032,China)
出处
《检验医学与临床》
CAS
2024年第11期1528-1532,共5页
Laboratory Medicine and Clinic
基金
云南省科技厅科技计划项目(202201AY070001-058)。
