摘要
【目的】探究香港牡蛎(Crassostrea hongkongensis)锌结合肽(ZnPE)在热环境、酸碱环境中的稳定性及跨Caco-2细胞模型的吸收转运机制,为锌补剂开发提供理论依据。【方法】以牡蛎锌螯合肽(Zn-PE)和ZnSO4为对照,以总锌、锌离子的溶解率为评价指标,在40、50、60、70和80℃以及pH值为2、4、6、8、10条件下,研究ZnPE的热稳定性和酸碱稳定性;并建立Caco-2细胞模型,研究ZnPE在跨Caco-2细胞模型的吸收率、生物利用率和转运途径;通过液质联用法研究ZnPE跨Caco-2细胞模型后的序列变化。【结果】不同温度中ZnPE与Zn-PE的总锌、锌离子溶解率无显著差异(P>0.05),两者均可保持较高的稳定性。在酸性环境(pH=2)中,ZnPE和Zn-PE中总锌溶解率均在90%以上,而锌离子有一定程度的释放,溶解率分别为22.30%、33.45%,两者稳定程度降低,但ZnPE稳定性显著高于Zn-PE(P<0.05)。在碱性环境(pH=8)中,ZnPE和Zn-PE均保持稳定。ZnPE可完整、稳定的通过Caco-2细胞模型,且其锌的吸收率为74.37%、生物利用率为76.40%,显著高于Zn-PE、ZnSO_(4)(P<0.05)。ZnPE中的锌主要以肽的形式通过细胞旁路和转胞吞作用的方式跨Caco-2细胞模型转运。转运后鉴定出8种锌结合肽,其中的5种和转运前一致,表明ZnPE在吸收转运中可保持较高的稳定性。【结论】ZnPE可在热环境、酸碱环境中及跨Caco-2细胞模型转运时保持较高的稳定性。
【Objective】To investigate the stability of oyster(Crassostrea hongkongensis)zinc-binding peptides(ZnPE)in thermal and acidic or alkaline environments and the mechanism of absorption and transport across the Caco-2 cell monolayers model,and the conclusions may provide theoretical basis for the development of zinc supplements.【Methods】Using oyster zinc-chelating peptides(Zn-PE)and ZnSO4 as control,the stability of ZnPE was evaluated by the solubility indexes of total Zn and Zn ions at 40,50,60,70 and 80℃,and at pH values of 2,4,6,8,and 10;a Caco-2 cell monolayer model was established to study the absorption rate,bioavailability,and transport pathway of ZnPE across Caco-2 cell monolayer model.The sequence changes of ZnPE across Caco-2 cell monolayers were investigated by liquid chromatography-mass spectrometry(LC-MS/MS).【Results】Under different temperatures,there was no significant difference in the solubility rates of total zinc and zinc ions in ZnPE and Zn-PE(P>0.05),and both of them could maintain a high stability.In acidic environment(pH=2),the solubility rate of total zinc in both ZnPE and Zn-PE was above 90%,while there was a slight release of zinc ions with the solubility rate of 22.30%and 33.45%,respectively,and the stability of both of them was reduced but the stability of ZnPE was significantly higher than that of Zn-PE(P<0.05).While in alkaline environment(pH=8),both ZnPE and Zn-PE remained stable.The ZnPE could completely and stably pass through Caco-2 cells monolayers model,and its zinc absorption was 74.37%and bioavailability was 76.40%,which were significantly higher than that of Zn-PE and ZnSO_(4)(P<0.05).The zinc in the ZnPE was transported across the Caco-2 cell monolayers model mainly in the form of peptides through cell bypass by means of transcytosis.Eight zinc-binding peptides were identified after transport,five of which were consistent with those before transport,suggesting that ZnPE can maintain high stability in absorption and transport.【Conclusion】ZnPE can maintain high s
作者
杜冉
陈忠琴
谭明堂
高加龙
林海生
郑惠娜
曹文红
DU Ran;CHEN Zhongqin;TAN Mingtang;GAO Jialong;LIN Haisheng;ZHENG Huina;CAO Wenhong(College of Food Science and Technology,Guangdong Ocean University/National Research and Development Branch Center for Shellfish Processing(Zhanjiang)/Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety/Guangdong Provincial Engineering Technology Research Center of Seafood/Guangdong Province Engineering Laboratory for Marine Biological Products/Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution,Zhanjiang 524088,China)
出处
《广东海洋大学学报》
CAS
CSCD
北大核心
2024年第3期103-111,共9页
Journal of Guangdong Ocean University
基金
湛江市农业技术攻关项目(2022A05038)。
关键词
牡蛎
锌结合肽
稳定性
CACO-2细胞
吸收转运
oyster
zinc-binding peptides
stability
Caco-2 cells
absorption and transport
