摘要
大黄鱼(Larimichthys crocea)作为我国产量最高、养殖规模最大的海水养殖鱼类,长时间不合理的饥饿易导致养殖效率低下,因此,需要合理控制越冬禁食时间。为了探究饥饿对大黄鱼的影响,在水温9.0~14.3℃条件下进行了饥饿0、8、16周的越冬养殖实验,研究了低温与饥饿双重胁迫下的大黄鱼生长、体成分、抗氧化酶活力以及相关基因的表达。结果显示,随着饥饿时间的延长,大黄鱼的肝体比(HSI)、脏体比(VSI)、肌肉和全鱼的粗脂肪和粗蛋白以及肝糖原含量显著降低(P<0.05),肌糖原含量无显著变化(P>0.05)。肝脏抗氧化酶丙二醛(MDA)活性先升高后降低;谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性显著降低(P<0.05),甘油三酯(TG)含量显著上升(P<0.05)。转录组分析结果显示,相比对照组,饥饿后差异基因显著下调,GO分析发现,饥饿后差异基因主要富集在脂质代谢过程、脂肪酸生物合成和补体激活等方面。聚类分析表明,饥饿16周后,脂肪代谢通路中肉碱棕榈酰转移酶1 (CPT1)、线粒体三功能酶β亚基(HADHB)、肉碱棕榈酰转移酶2 (CPT2)基因的相对表达水平升高,而脂肪酸延长酶(HACD)、脂肪酸合成酶(FASN)、5-氨基乙酰丙酸合成酶(ALAS)基因的相对表达水平下降;免疫代谢通路中丝氨酸蛋白酶抑制剂(SERP)、纤维蛋白原-1 (FGG-1)、纤维蛋白原-3 (FGG-3)、纤维蛋白原β (FGB)基因的相对表达水平也降低,qRT-PCR也证实了以上结果。综上所述,越冬期间,长时间的饥饿将抑制大黄鱼的生长和免疫相关基因的表达,促进其肝脏抗氧化能力和脂质代谢相关基因的表达。
Being the most productive and extensively farmed marine species in China,prolonged and unjustified starvation can drastically reduce the aquaculture efficiency of large yellow croakers(Larimichthys crocea).Hence,it is imperative to regulate the duration of winter fasting in a reasonable manner.An experiment was conducted during winter to explore the effects of fasting on large yellow croaker.The aquaculture study took place at temperatures ranging from 9.0 to 14.3°C for durations of 0,8,and 16 weeks.This investigation scrutinized the impacts of low temperature and starvation on the growth,body composition,antioxidative enzyme activity and gene expression of large yellow croaker.Findings indicated that the hepato-somatic index(HSI),viscera-somatic index(VSI),crude fat,crude protein in muscles and whole fish,and liver glycogen content all significantly decreased(P<0.05)with prolonged starvation,whereas muscle glycogen content remained relatively unchanged(P>0.05).The liver antioxidative enzyme malondialdehyde(MDA)exhibited an initial increase followed by a decrease;the activities of glutathione peroxidase(GSH-Px)and catalase(CAT)noticeably decreased(P<0.05),while triglyceride(TG)activity significantly increased(P<0.05).The transcriptome analysis results showed that the differentially expressed genes were significantly down-regulated after starvation compared to the control group.GO analysis revealed that the differentially expressed genes after starvation were mainly enriched in processes such as lipid metabolism,fatty acid biosynthesis,and complement activation.Cluster analysis indicated that after 16 weeks of starvation,the relative expression levels of carnitine palmitoyltransferase 1(CPT1),3-ketoacyl-CoAthiolase(HADHB),and carnitine palmitoyltransferase 2(CPT2)genes in the fatty acid metabolism pathway were increased,while the relative expression levels of genes for fatty acid elongase(HACD),fatty acid synthase(FASN),and 5-aminolevulinate synthase(ALAS)were decreased.The relative expression levels of genes in
作者
黄玲
尹恒
邱浩宇
王涵颖
陶晨智
冉志强
许嘉航
王萍
HUANG Ling;YIN Heng;QIU Hao-Yu;WANG Han-Ying;TAO Chen-Zhi;RAN Zhi-Qiang;XU Jia-Hang;WANG Ping(College of Marine Science and Technology,Zhejiang Ocean University,Zhoushan 316022,China;National Marine Facilities Aquaculture Engineering Technology Research Center,Zhejiang Ocean University,Zhoushan 316022,China)
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2024年第3期765-774,共10页
Oceanologia Et Limnologia Sinica
基金
国家自然科学基金项目,47176119号,32273162号
舟山市科技计划项目,2020C21008号
浙江海洋大学科研启动项目,11105090718号。
关键词
大黄鱼
低温与饥饿胁迫
生长性能
体成分
肝脏抗氧化能力
基因表达
Larimichthys crocea
cold and starvation stress
growth performance
body composition
liver antioxidative capacity
gene expression
