摘要
采用组织分离法和单孢子纯化法,从拟石莲花属多肉植物品种‘碧桃’(Echeveria cv.‘Peach Pride’)疑似腐烂病的叶片组织获得病原菌,通过形态学和分子序列特征的鉴定以及致病性测定,明确病原菌为尖孢镰刀菌(Fusarium oxysporum)。测定尖孢镰刀菌的主要致病物质,发现该病原菌具有较强的细胞壁降解酶活性,并具有一定的产毒素(镰刀菌酸)能力,说明细胞壁降解酶类和毒素是该病原菌侵入、定殖和致病的主要因素。
The fungal pathogen was obtained from the leaves of Echeveria cv.‘Peach Pride’with suspected rot disease based on tissue separation and single spore separation.According to morphological observation,molecular identification and pathogenicity test,this pathogen was identified as Fusarium oxysporum(F.oxysporum).The main pathogenic substances of F.oxysporum was determined,it was found that the pathogen has strong degrading enzyme activity on cell wall and the ability to produce toxins(fusaric acid),indicating that cell wall degrading enzymes and toxins are the main factors for the pathogen’s invading,colonizing and pathogenesis.
作者
刘爽
张廷萍
薛治峰
刘杨
王婧
钱亚新
秦世雯
LIU Shuang;ZHANG Tingping;XUE Zhifeng;LIU Yang;WANG Jing;QIAN Yaxin;QIN Shiwen(School of Institute of Resource Plants,Yunnan University,Kunming,Yunnan 650500,China;College of Plant Protection,Northwest A&F University,Yangling,Shanxi 712100,China)
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2024年第2期65-70,共6页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家自然科学基金项目(32060593)
云南省基础研究计划面上项目(202101AT070021)。
