摘要
为明确黄蜀葵枯萎病的病原菌种类,本研究利用形态学和分子生物学方法对病原菌进行鉴定和柯赫氏法则验证,并探究其生物学特性和对杀菌剂的敏感性。结果表明,分离得到的致病病原菌为木贼镰孢菌(Fusarium equiseti)。病原菌生长最适温度范围为25~30℃,致死条件为57℃处理10 min。在碱性和光照条件下,最佳碳源为葡萄糖和蔗糖,最佳氮源为牛肉浸膏;在pH值为5和光照交替条件下,最适产孢的碳源为山梨醇,氮源为蛋白胨。杀菌剂敏感性分析结果表明,咪鲜胺、多菌灵、苯醚甲环唑、吡唑醚菌酯和甲基硫菌灵对菌丝生长均有抑制作用,其中抑制效果最好的杀菌剂是咪鲜胺,EC_(50)为0.0712 mg·L^(-1)。本研究结果为黄蜀葵枯萎病的防治提供了理论依据。
In order to explore the pathogenic species of the wilt disease in A.manihot,the pathogen was identified by morphological and molecular biology methods and validated by Koch’s postulates,and investigated its biological characteristics and sensitivity to fungicides.The results were as follows:the isolated pathogen was identified as Fusarium equiseti.the optimal growth temperature of the pathogen was 25-30℃,and the lethal temperature was 57℃ for 10 minutes.Under alkaline and light conditions,the best carbon sources were glucose and sucrose,whereas the best nitrogen source was beef extract.Under pH 5 and alternating light conditions,the carbon source most conducive to sporulation was sorbitol,with peptone being the best nitrogen source.The analysis of fungicides sensitivity indicated that prochloraz,carbendazim,difenoconazole,pyraclostrobin and methylthiophanate had inhibitory effects,and the best fungicide was prochloraz,median effect dose(EC_(50))was 0.0712 mg·L^(-1).This research provides a theoretical basis for prevention and control of wilt disease in A.manihot.
作者
唐健林
程亮亮
叶磊
檀根甲
TANG Jianlin;CHENG Liangliang;YE Lei;TAN Genjia(Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes,School of Plant Protection,Anhui Agricultural University,Hefei,Anhui 230036)
出处
《核农学报》
CAS
CSCD
北大核心
2024年第7期1416-1423,共8页
Journal of Nuclear Agricultural Sciences
基金
安徽省高校学科(专业)拔尖人才学术资助重点项目(gxbjZD2016018)。
关键词
木贼镰孢菌
黄蜀葵
生物学特性
毒力测定
Fusarium equiseti
Abelmoschus manihot
biological characteristics
toxicity test
