摘要
为研究穿心莲HPLC指纹图谱及体外抗氧化作用的谱效关系,构建穿心莲抗氧化作用网络,整合谱效关系和网络药理学数据,预测穿心莲抗氧化作用的物质基础。采用HPLC法建立10批次穿心莲的指纹图谱;测定穿心莲对大鼠肝细胞(BRL-3A)中活性氧(ROS)及丙二醛(MDA)含量的影响;采用灰色关联分析法进行谱效关联分析,初步确定抗氧化成分;结合网络药理学,预测抗氧化候选成分;根据分析结果,探究穿心莲抗氧化成分。结果:本研究建立了穿心莲指纹图谱,共标定11个共有峰;根据灰色关联度大小排序,9、7、10、5、1、6、8、3号共有峰与BRL-3A细胞ROS含量有较高关联度;9、7、10、5、1、6、8、3号共有峰与BRL-3A细胞MDA含量有较高关联度;网络药理学推测穿心莲抗氧化成分主要包括14-脱氧穿心莲内酯、新穿心莲内酯、穿心莲苷A等25个;整合分析确定穿心莲抗氧化成分为共有峰9、7、10、5、1、6、8、3代表的成分及14-脱氧穿心莲内酯、新穿心莲内酯等。综上,穿心莲抗氧化药效是多种成分共同作用的结果,穿心莲的活性成分可能主要通过细胞凋亡途径,作用于丝/苏氨酸蛋白激酶(AKT1)、内皮生长因子受体(EGFR)、半胱氨酸蛋白酶3(CASP3)等靶点发挥其药理作用。本研究可对穿心莲抗氧化的物质基础研究提供参考。
This study was to explore the HPLC fingerprint of Andrographis paniculata and the relationship between its spectrum and efficacy in vitro,to construct a network of the antioxidant effect of Andrographis paniculata,and to integrate the spectrum and the network pharmacol⁃ogy information,for purpose of predicting the material basis of oxidation resistance of Andrographis paniculatafor.The fingerprints of 10 bat⁃ches of Andrographis paniculata were established by HPLC,and the contents of ROS and MDA in BRL-3A cells treated with Andrographis paniculate were determined using a microplate reader,and the antioxidant ingredients were preliminarily determined by grey correlation analy⁃sis.The candidate components of antioxidants were predicted in combination of network pharmacology in order to analyze the antioxidant com⁃ponents of Andrographis paniculata.The results were that the fingerprint of Andrographis paniculata was established and 11 common peaks were calibrated.The grey correlation analysis showed that the common peaks of 9,7,10,5,1,6,8 and 3 had a higher relevance with the ROS content of BRL-3A cells,and that the common peaks of 9,10,5,7,6,8,1 and 3 had a higher relevance with the MDA content of BRL-3A cells.According to the network pharmacology,25 components,including 14-deoxyandrographolide,neo-andrographolide,and rographidine A,were speculated to be the antioxidant components of Andrographis paniculata.The integrated analysis suggested that the an⁃tioxidant components of Andrographis paniculata were 9,7,10,5,1,6,8 and 3 common peaks,and 14-deoxyandrographolide,neo-an⁃drographolide,and so on.Taken together,the antioxidant effect of Andrographis paniculatais was believed to be attributed to the interaction of various components.The active ingredients of Andrographis paniculata might exert their pharmacological effects mainly through the apoptotic pathway,acting on AKT1,EGFR,CASP3 and other targets.The results provided reference for future research on the material basis of the antioxidant effect of An
作者
杨黎宇
李家伟
李燕玲
胡诗雨
李健
YANG Liyu;LI Jiawei;LI Yanling;HU Shiyu;LI Jian(College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《畜牧与兽医》
CAS
北大核心
2024年第6期55-62,共8页
Animal Husbandry & Veterinary Medicine
基金
福建省科技重大专项资助项目(2021NZ029023)
福建农林大学科技创新专项基金(CXZX2016013)。
关键词
穿心莲
抗氧化
HPLC指纹图谱
谱效关系
网络药理学
BRL-3A细胞
Andrographis paniculat
oxidation resistance
HPLC fingerprint
spectral efficacy relationship
network pharmacology
BRL-3A cells
