摘要
目的:探究L-苏糖酸镁(L-TAMS)对组胺和氯喹引起的小鼠急性瘙痒及瘙痒敏化的影响。方法:将56只小鼠随机平均分为7组,对照组(A组)、组胺瘙痒敏化组(B组)、氯喹瘙痒敏化组(C组)、L-TAMS+组胺瘙痒敏化组(D组)、L-TAMS+氯喹瘙痒敏化组(E组)、组胺瘙痒敏化+L-TAMS组(F组)、氯喹瘙痒敏化+L-TAMS组(G组)。A组生理盐水注射对照,B、D、F组采用组胺(2 mg/ml,50μl)连续注射4天瘙痒敏化造模,C、E、G组采用氯喹(2 mg/ml,50μl)连续注射4天瘙痒敏化造模;D、E组在敏化造模前预防性口服L-TAMS一周,F、G组小鼠在敏化后口服L-TAMS一周。每次注射组胺和氯喹后进行瘙痒行为学测试。结果:与B组相比,D组第1天注射组胺后的搔抓次数没有统计学意义(P>0.05);D组第3、4天注射组胺后的搔抓次数明显减少(P<0.01)。与B组相比,D、F组第11天注射组胺后的搔抓次数明显减少(P<0.001)。与C组相比,E组第1天注射氯喹后的搔抓次数没有统计学意义(P>0.05);E组第4天注射氯喹后的搔抓次数明显减少(P<0.01)。与C组相比,E、G组第11天注射氯喹后的搔抓次数明显减少(P<0.05,P<0.01)。结论:口服L-TAMS能有效预防和逆转小鼠被组胺和氯喹诱导的瘙痒敏化。
Objective:To explore the effects of L-Threonate Magnesium(L-TAMS)on acute itching and sensitization induced by histamine and chloroquine in mice.Methods:56 mice were randomly divided into 7 groups:Control(Group A),Histamine Sensitization(Group B),Chloroquine Sensitization(Group C),L-TAMS+Histamine Sensitization(Group D),L-TAMS+Chloroquine Sensitization(Group E),Histamine Sensitization+L-TAMS(Group F)and Chloroquine Sensitization+L-TAMS(Group G).Group A received saline injections as a control.Groups B,D and F were sensitized with histamine(2 mg/ml,50μl)injections for 4 days,while Groups C,E and G with chloroquine(2 mg/ml,50μl)for 4 days.Groups D and E were pre-treated with L-TAMS for one week before sensitization and Groups F and G after sensitization.Pruritus behavior tests were conducted after each histamine and chloroquine injection.Results:Compared with Group B,Group D showed no significant difference in scratching frequency on the first day(P>0.05),but a significant reduction on the third and fourth days(P<0.01)and a marked reduction on the eleventh day(P<0.001).Compared with Group C,Group E showed no significant difference on the first day(P>0.05),but a significant reduction on the fourth day(P<0.01)and a marked reduction on the eleventh day(P<0.05,P<0.01)for both Groups E and G.Conclusion:Oral L-TAMS effectively prevents and reverses itch sensitization induced by histamine and chloroquine in mice.
作者
黄卓
乔琬华
周鑫
HUANG Zhuo;QiAO Wan-hua;ZHOU Xin(Hubei Polytechnic Institute,Xiaogan,Hubei 432000,China;Guangdong Provincial People's Hospital,Guangdong,Guangzhou 510000,China)
出处
《湖北职业技术学院学报》
2024年第2期109-112,共4页
Journal of Hubei Polytechnic Institute
基金
湖北职业技术学院校级重点项目“L-苏糖酸镁对于组胺和氯喹引起的急性瘙痒及瘙痒敏化的抑制作用的研究”(项目编号:2022A02)的阶段性研究成果。
