摘要
目的:利用网络药理学研究牛膝何首乌联用抗骨质疏松的作用机制。方法:运用中药系统药理学数据库与分析平台(TCMSP)与文献检索筛选何首乌、牛膝两味中药所含的活性成分。利用Disgent数据库搜索与骨质疏松相关的蛋白靶点;利用Pubchem平台,预测何首乌、牛膝所含活性成分所作用的人类蛋白靶点。分析牛膝何首乌药的交集靶点,并将交集靶点进行蛋白网络分析,构建药物-成分-靶点的网络图。通过微生信平台对关键基因作用的靶向细胞进行富集分析。结果:确定何首乌成分5个,牛膝成分10个,二者共有成分1个,获得药对与疾病交集基因35个;PPI蛋白分析获得10个主要作用成分;绘制GO分析可视图、KEGG分析可视图并对其进行解析,解析结果表明何首乌牛膝主要成分通过抑制骨吸收,诱导骨形成等通路来减轻骨质疏松症的发展。结论:牛膝何首乌药对抗骨质疏松的作用机制可能是通过靶点与共有通路完成的,并且该药对可通过多通路、多靶点、多成分、多层次发挥对骨质疏松症的治疗作用。
Objective:To learn and use network pharmacology to study the mechanism of combined use of Achyranthes bidentata and Polygonum multiflorum against osteoporosis.Methods:The traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP)and literature search were used to screen the active ingredients contained in Polygonum multiflorum and Achyranthes bidentata.Use the Disgent database to search for protein targets related to osteoporosis;use the Pubchem platform to predict the human protein targets acted on by the active ingredients contained in Polygonum multiflorum and Achyranthes bidentata.Analyze the intersection targets of Achyranthes bidentata and Polygonum multiflorum,and conduct protein network analysis on the intersection targets to construct a network diagram of drug-ingredient-target.Enrichment analysis of targeted cells affected by key genes was carried out through the Weishengxin platform.Results:5 components of Polygonum multiflorum and 10 components of Achyranthes bidentata were identified,and 1 component was shared between them.35 genes intersecting drug pairs and diseases were obtained;10 main active components were obtained through PPI protein analysis;GO analysis visual diagram and KEGG analysis were obtained.View and analyze it.The analysis results show that the main components of Polygonum multiflorum Achyranthes bidentata can reduce the development of osteoporosis by inhibiting bone resorption and inducing bone formation.Conclusion:The mechanism of action of Achyranthes bidentata and Polygonum multiflorum against osteoporosis may be through targets and shared pathways,and the drug can exert its therapeutic effect on OP through multiple pathways,multiple targets,multiple components,and multiple levels.
作者
石安骐
石舒骐
林磊婷
孙艳涛
SHI Anqi;SHI Shuqi;LIN Leiting;SUN Yantao(Liaoning University of Traditional Chinese Medicine,Liaoning Dalian 116600,China)
出处
《中医药临床杂志》
2024年第5期912-917,共6页
Clinical Journal of Traditional Chinese Medicine
基金
辽宁中医药大学中医脏象理论及应用国家教育部重点实验室开放基金课题(zyzx1905)
辽宁中医药大学“大学生创新创业训练计划资助”(X202110162036)。
关键词
配伍机制
网络药理学
何首乌-牛膝
药对
骨质疏松症
Compatibility mechanism
Network pharmacology
Polygonum multiflorum-Achyranthes bidentata
Drug pair
Osteoporosis
